Product Introduction
Cell proliferation detection is a fundamental experimental method for assessing cell health, genetic toxicity, and the efficacy of anti-tumor drugs. The most accurate method for detecting cell proliferation is the BrdU method. The EdU method is a revolutionary breakthrough over the BrdU method. EdU (5-ethynyl-2'-deoxyuridine) is a pyrimidine analog that integrates into DNA during the DNA synthesis phase. The detection is based on a "click" reaction, a covalent reaction catalyzed by copper between azide compounds and alkynes. The EdU labeling method is rapid and efficient, easy to use. It only requires formaldehyde fixation and Triton X-100 permeabilization to allow the detection reagent to enter the cells. A small amount of azide dye can effectively label the integrated EdU. In contrast, the BrdU method requires DNA denaturation (such as acid denaturation, heat denaturation, or DNase digestion) to expose BrdU for antibody binding. This kit includes all the components required for EdU detection and can be used for the proliferation detection of in vitro cultured cells.
Basic Information
Product name | EdU Cell Proliferation Assay Kit (Fluorescein 555,Orange-Red) |
Sizes | 20T/100T/500T |
Storage | -20℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Ex/Em | 555/565 nm |
Product Components
components | 2~20 T | 10~100 T | 50~500 T | Storage Temperature After Opening | stability |
A. 10 mM EdU | 40 µL | 200 µL | 1 mL | -20 ℃ | After opening, different components are stored at the specified temperature, such as subsequent storage-20℃, which is also possible. |
B. 555A Azide | 10 µL | 50 µL | 250 µL | -20 ℃，protect form light | |
C. 10 × Click-iT EdU reaction buffer | 200 µL | 1 mL | 5 mL | 2~8 ℃ | |
D. CuSO4 | 100 µL | 500 µL | 2 × 1.25 mL | 2~8 ℃ | |
E. Click-iT EdU Buffer additives | 6 mg | 30 mg | 150 mg | 2~8 ℃ | |
F. Hoechst 33342 | 5 µL | 25 µL | 125 µL | 2~8 ℃ |
Specifications: For fluorescence microscopy applications, the maximum number of uses per kit is the highest specified for the 96-well plate culture system, such as the 20T kit which can process 20-well samples (specific volumes for different containers are referenced in Table 2). For flow cytometry applications, the minimum number of uses per kit is the lowest specified for the same system, with the 20T kit designed to process 2 samples.
Notes
1.Before use, centrifuge the product to the bottom of the tube immediately before proceeding with subsequent experiments.
2. EdU (10 mM) should be aliquoted according to experimental requirements for initial use and stored at-20°C.
3. This product should not be tested simultaneously with TUNEL reagent kits, as the-OH groups in EdU's structure may interfere with TUNEL's reaction process.
4. Since copper ions can disrupt actin structure and affect digoxin detection, this product is incompatible with Phalloidin (digoxin).
5. After opening, store components according to the instructions provided in the package.
6. Fluorescent dyes are prone to quenching; ensure light protection during operation to minimize fluorescence quenching.
7. For safety, wear lab coats and disposable gloves during handling.
8. This product is for research purposes only and must not be used for clinical diagnosis or treatment.
This product is for research use only！
