Polycomb Group
The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell-cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, Eed-Ezh2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinates histone H2A on Lys119. PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of an additional catalytic subunit RING1B (also RING2 or RNF2). PcG proteins play an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and are required for maintenance of adult hematopoietic and neural stem cells, as well as embryonic stem cells.
Targeting of PRCs
Relevant Antibodies
| Catalog# | Product Name | Application | Reactivity |
|---|---|---|---|
| AMRe21550 | Polycomb Protein SUZ12 Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse,Rat |
| AMRe21173 | EZH2 Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse,Rat |
| AMM81219 | Ring1 Mouse Monoclonal Antibody | WB,IHC,FC,ELISA | Human |
| AMRe03274 | RING2 Rabbit Monoclonal Antibody | WB,IHC-P | Human |
| AMRe07586 | Bmi1 (10S4) Rabbit Monoclonal Antibody | WB,IHC-P,ICC/IF,IF-P | Human,Rat |
| AMRe84830 | TriMethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody | WB,IP,IHC,ICC | Human,Rat |
| APS0631 | HRP-conjugated Polyclonal Goat Anti-Mouse IgG(H+L) Secondary Antibody | ELISA,WB,Dotblot | Mouse |
| AMRe80004 | GAPDH (12R9) Rabbit Monoclonal Antibody | WB,ELISA | Human,Mouse,Rat,Rabbit,Dog,Monkey |
Related Products
- Antibody Labeling Kit
- Western Blot Kits
- Super-sensitive ECL chemiluminescent reagent
- IHC Kit
- TSA mIHC Kits
References
- Boyer, L.A. et al. (2006) Nature 441, 349-53.
- Lee, T.I. et al. (2006) Cell 125, 301-13.
- Park, I.K. et al. (2003) Nature 423, 302-5.
- Molofsky, A.V. et al. (2003) Nature 425, 962-7.
- Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
- Wang, H. et al. (2004) Nature 431, 873-8.
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
- Jacobs, J.J. et al. (1999) Nature 397, 164-8.
- Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.
- Di Croce L, Helin K. (2013). Nature structural & molecular biology, 20(10): 1147-1155.
 | Voisey Voisey is a technical support specialist at EnkiLife, proficient in immunology and cell biology. She is committed to providing customers with professional and efficient technical support. Additionally, she is involved in research on customers' fields of study and designs highly cost-effective solutions for them. |