Summary
| Production Name | β-actin Monoclonal Antibody(5B7) |
| Application | IF,WB,IHC-p,Elisa |
| Reactivity | Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Insect |
| Purification | Affinity Chromatography |
| Gene Name | β-actin |
| Immunogen | Synthetic Peptide of β-actin |
| Specificity | The antibody detects endogenous β-actin protein. |
| Dilution Ratio | IF-P/IF-F/IF-ICC 1:50-200, WB 1:5000, IHC-P 1:200 |
| Host | Mouse |
| Isotype | IgG |
| Buffer | Liquid in PBS,pH 7.4, containing 50% glycerol，2% protective protein and New type preservative N. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Background | beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues. |
