Product Introduction
Alcian Blue, also known as Alcian Blue 8GX, is a copper-containing phthalocyanine dye originally used for textile fiber staining. This cationic dye binds to acidic groups, forming insoluble complexes with anionic groups such as carboxyl and sulfate groups in tissues. The structure of Alcian Blue consists of a central copper-containing phthalocyanine ring linked to four isothiouronium groups via thioether bonds. The isothiouronium groups are moderately basic, giving the dye a positive charge. At pH 2.5, carboxyl groups in tissues are ionized and carry a negative charge, forming electrostatic bonds with the positively charged dye, thus staining acidic mucosubstances such as sialomucins and sulfomucins.
EnkiLife Alcian Blue Staining Solution (pH 1.0) utilizes different pH values to distinguish types of mucosubstances: At pH 2.5: carboxyl groups are well stained, sulfated mucins are poorly stained. At pH 1.0: sulfate groups are ionized and carry a negative charge, allowing selective staining of sulfated mucosubstances. Neutral mucins (e.g., in gastric mucosa and Brunner’s glands) do not react with Alcian Blue.
Basic Information
Product name | Alcian Blue Staining Kit, pH 1.0 |
Sizes | 50 mL |
Storage | 2-8 ℃，keep away from light |
Shipping | Shipped with ice pack |
Validity | 6 months |
Product Components
Components | 3x 50mL |
Reagent (A): Alcian Acidification Solution | 50 mL |
Reagent (B): Alcian Staining Solution | 50 mL |
Reagent (C): Nuclear Fast Red Staining Solution | 50 mL |
Notes
1. Use 10% neutral formalin as fixative.
2. This method can distinguish sulfated mucins and proteoglycans at pH = 1.0.
3. After opening, use the reagent within 6 months. Always tighten the cap after use to prevent evaporation or degradation.
4. For your safety and health, wear a lab coat and disposable gloves.
5. Use the reagent promptly after opening to ensure optimal performance.
