Product Introduction
Eosin Y, also known as eosin, is a synthetic xanthene dye, appearing as a pink or rose-colored powder. Its molecular formula is C₂₀H₆O₅Br₄Na₂, with a molecular weight of 691.86. Eosin Y is commonly used in combination with hematoxylin for staining normal and pathological tissue structures. The aqueous form of Eosin Y contains a quinoid benzene ring chromophore and two acidic auxochrome groups (R–COONa and R–ONa) that form sodium salts. In aqueous solution, this acid dye dissociates into a negatively charged colored ion (R–COO⁻, R–O⁻) and a positively charged sodium ion (Na⁺). During staining, the negatively charged colored portion of Eosin Y binds via ionic interactions to positively charged components in the tissue, resulting in visible coloration.
EnkiLife’s aqueous Eosin Y staining solution is prepared using high-quality imported raw materials and a proprietary preservative. The formulation is user-friendly and avoids the use of toxic reagents such as mercury or methanol. This product is suitable for staining tissue sections and cultured cells, and is typically used in conjunction with hematoxylin. It can also be applied following immunofluorescence or immunohistochemical staining—either as a counterstain after immunostaining, or prior to additional fluorescent labeling. The solution can be reused until staining efficacy declines. After staining, the cytoplasm appears pink to red.
Basic Information
Product name | Eosin Y Staining Kit, Aqueous |
Sizes | 500 mL |
Storage | RT |
Shipping | RT |
Validity | 12 months |
Notes
1. For dehydration, clearing, and mounting, users must prepare xylene or xylene substitute and mounting medium (e.g., neutral balsam).
2. For paraffin sections, users must prepare 90% ethanol, absolute ethanol, and xylene or substitute.
3. Wear appropriate personal protective equipment (lab coat and gloves) during operation.
4. Use the reagent promptly after opening to ensure optimal performance.
