Product Introduction

Citric acid synthetase, also known as citrate condensate enzyme, plays a crucial role in the tricarboxylic acid cycle (also known as the citric acid cycle) because the reaction it catalyzes is a key step. The tricarboxylic acid cycle is a ubiquitous metabolic pathway in aerobic organisms, serving as the hub and final metabolic pathway connecting the metabolism of the three major nutrients: carbohydrates, lipids, and amino acids.The tricarboxylic acid cycle (TCA cycle), also known as the citric acid cycle or the Krebs cycle, is a ubiquitous metabolic pathway in aerobic organisms. It is named after citric acid, a compound containing three carboxyl groups, which is one of the main intermediate metabolites in this cycle. Alternatively, it can be named the Krebs cycle after its discoverer, Hans-Adolf Krebs. The TCA cycle is essential for the metabolism of three major nutrients. It is the final metabolic pathway of carbohydrates, lipids, and amino acids, and also the hub connecting the metabolism of carbohydrates, lipids, and amino acids.

Principle

In the tricarboxylic acid cycle, the reactant glucose or fatty acid is converted into acetyl-CoA. This "activated acetic acid" (one molecule of coenzyme linked to an acetyl group) breaks down in the cycle to produce the final product carbon dioxide and undergoes dehydrogenation. The proton is then transferred to the coenzymes nicotinamide adenine dinucleotide (NAD+) and flavin adenine (FAD), resulting in NADH+ H+ and FADH2. NADH+ H+ and FADH2 then... It continues to be oxidized in the respiratory chain into NAD+ and FAD, and produces water. This regulated "burning" generates ATP, providing energy. The mitochondria of eukaryotes and the cytoplasm of prokaryotes are the sites of the tricarboxylic acid (TCA) cycle. It is a step in the respiratory process, but in aerobic organisms, it precedes the respiratory chain. Anaerobic organisms initially follow the same pathway, breaking down high-energy organic compounds, such as through glycolysis, but do not subsequently undergo the TCA cycle; instead, they proceed with fermentation, a process that does not require oxygen.In the first step of the triacid cycle, catalytic reaction involves the ethyl group of acetyl-CoA combining with the ketone group of oxaloacetate to form citrate-CoA. This catalyzes subsequent high-energy sulfate bond hydrolysis, releasing coenzyme A and yielding citrate. Citrate synthase is a regulatory enzyme; its substrates, acetyl-CoA and oxaloacetate, are its activators, while NADH and succinyl-CoA are its inhibitors.

Storage

This kit is stored under the specified conditions upon receipt and has a shelf life of 6 months.

Components

Notes 

This product is for research use only and must not be used for clinical diagnosis. Do not take it.