Product Introduction

Diamine oxidase (DAO) is a copper-containing amine oxidase widely distributed in tissues such as the intestinal mucosa, placenta, kidneys, and thymus. DAO catalyzes the oxidative deamination of diamine substrates such as histamine, putrescine, and cadaverine, producing the corresponding aldehydes, ammonia, and hydrogen peroxide. When tissue cells are damaged or cell membrane permeability is altered, DAO is released into the bloodstream, leading to increased serum DAO activity. However, under specific physiological or pathological conditions (such as placental insufficiency), serum DAO activity may abnormally decrease. This reagent is used to measure serum DAO activity, primarily for monitoring placental function in pregnant women: DAO levels gradually increase before labor; a sudden decrease is a sign of miscarriage, prompting preventative measures. Literature reports elevated serum DAO levels in gastrointestinal diseases and enteritis, as well as in colon cancer and ascites.

Principle

The NADH decrease rate per minute (-ΔA/min) was measured at a wavelength of 340 nm, and its activity was calculated.

Storage

The reagent kit has a shelf life of 3 months.

Notes 

1.Technical specifications: Blank absorbance A at 340nm >0.5, batch-to-batch precision<10%, linear range 0–100 U/L;
2.This product is for research use only and must not be used for clinical diagnosis. Do not take it.

Components