Product Introduction
ATPase is located on the cell membranes and organelle membranes of tissues, serving as a type of protease on biological membranes. It plays vital roles in substance transport, energy conversion and information transmission. A series of changes in ATPase activity occur under oxygen deficiency and certain disease conditions in the body. In addition, some genetic diseases are also related to the level of this enzyme activity.
Principle
ATPase can decompose ATP into ADP and inorganic phosphorus. The activity of ATPase can be determined by measuring the amount of inorganic phosphorus. Cu²⁺-ATPase is an enzyme that can be activated by potassium and inhibited by vanadate.
Storage
The kit has a validity period of 6 months.
Notes
1.It is recommended to use disposable laboratory test tubes for operation.
2.This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
3.This method features microscale detection, high sensitivity and rapid operation. Therefore, the test tubes used must meet strict requirements and be completely phosphorus-free. Disposable phosphorus-removed plastic tubes or centrifuge tubes are recommended. Preventing phosphorus contamination is critical to the success of the assay.
4.Once the phosphorus assay reagent is prepared, do not store it for too long. Its maximum shelf life is one day, and fresh preparation immediately before use is recommended. Keep it refrigerated at all times.
5.All vessels used for reagent preparation must be dedicated, including pipettes for sulfuric acid and containers for water.
6.It is recommended to first prepare the mixed solutions for test tubes and control tubes, then perform the assay following the simplified procedures for higher efficiency and accuracy.
Components
No. | Components | Size | Notes |
Reagent 1 | Liquid | 10 mL×2 | Store at 4°C |
Reagent 2 | Liquid | 10 mL×1 | Store at 4°C |
Reagent 3 | Liquid | 7 mL×1 | Store at 4°C |
Reagent 4 | Powder | 3 vials | Store at 4°C. Add 5 mL of double-distilled water to each powder vial and dissolve completely after use. After preparation, store below -20 °C for up to 7 days. Do not repeat freeze-thaw cycles. |
Reagent 5 | Powder | 1 vial | Store at 4°C. Add 5 mL of double-distilled water to each vial and dissolve completely (moderate heating is acceptable) before use. |
Reagent 6 | Powder | 1 vial | Store at 4°C. Pour the powder into the solvent and dissolve completely before use. |
Liquid | 3 mL×1 | ||
Reagent 7 | Liquid | 10 mL×1 | RT |
Reagent 8 | Powder | 3 vials | Store at 4°C. Add 40 mL of double-distilled water to each bottle of powder to dissolve completely before use. Store at 4°C. Discard if the solution darkens in color. |
Reagent 9 | Powder | 1 vial | Store at 4°C. Dissolve each bottle of powder with 100 mL of double-distilled water before use, and store at 4°C. |
Reagent 10 | 2.5 mol/L Sulfuric Acid | 100 mL×1 | RT |
Reagent 11 | 10 mmol/L Standard Phosphorus Stock Solution | 10 mL×1 | Store at 4°C. Dilute the stock solution with double-distilled water at a ratio of 1:19 before use (e.g., take 0.5 mL of the stock solution and dilute to 10 mL with double-distilled water) to prepare a 0.5 μmol/mL working standard phosphorus solution. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |