Product Introduction
Butyrylcholinesterase hydrolyzes butyrylcholine to produce choline and acetic acid. Choline reacts with sulfhydryl chromogenic reagent to form yellow TNB compounds. Colorimetric quantification is performed according to color intensity, and the content of hydrolyzed choline reflects the activity of cholinesterase.
Storage
The validity period of the kit is 3 months.
Notes
1.It is recommended to use disposable laboratory test tubes for operation.
2.This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
Components
No. | Components | Size | Notes |
Reagent 1 | Standard powder | 3 vials | Store at 4°C. 1 μmol/mL Standard Working solution preparation: Dissolve one vial of standard substance in 10 mL normal saline and mix well. Prepare freshly before use. |
Reagent 2 | Substrate | 2 vials | Store at 4°C. Preparation of Substrate Buffer: Add 20 mL normal saline to each vial of powder before use to prepare substrate buffer, which can be stored at 4°C for two weeks. |
Reagent 3 | Chromogenic | 3 mL | Store at 4°C. Preparation of Chromogenic Working Solution: Dilute the chromogenic stock solution with normal saline at a ratio of 1:9 before use to prepare the chromogenic working solution. Prepare as needed, or prepare 30 mL at a time. Store away from light at 2-8 °C for up to 3 months. |
Reagent 4 | Inhibitor | 1 bottle | Store at room temperature. Transfer it into an empty small plastic bottle after opening. |
Reagent 5 | Clearing agent | 6 mL | Store at room temperature. Precipitation or turbidity may occur in cold weather; heat at 37°C until clear before use. |
Reagent 6 | Normal saline | 60 mL×2 | Store at room temperature. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |