Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. The glucose content of DMEM medium was originally designed to be 1000 mg/L (low sugar type), and later it was developed to have a glucose content of 4500 mg/L (high sugar type). It is now widely used in the culture of various cells. This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for the culture of various types of cells, but does not contain proteins, lipids or any growth factors, so this product must be used with serum or serum-free additives.
Details
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;
3. This product is only used for research or further research, not for diagnosis and treatment.
4. It is not suitable for long time storage at room temperature.
5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.
