McCoy's 5A medium was designed by Thomas McCoy and others in 1959. It was originally used specifically for the culture of Novikoff Hepatoma cells. McCoy's 5A medium is a modified Basal Medium 5A. Unlike other culture media, McCoy's 5A contains reduced glutathione, bacterial peptone, and a high concentration of glucose. McCoy's 5A is widely used for the culture of various types of primary cells, such as bone marrow, skin, gums, kidneys, spleen, lungs, rat embryos, omentum, etc. In addition, McCoy's 5A medium is also used for tissue biopsy culture, cell line establishment, and the culture of some lymphocytes and cells that are difficult to culture.
Details
Precautions
1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;
3. This product is only used for research or further research, not for diagnosis and treatment.
4. It is not suitable for long time storage at room temperature.
5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.
