Product Introduction
Glycogen staining is one of the routine staining methods in pathology. In 1946, McManus first used the periodic acid–Schiff technique to visualize mucins. This method is commonly used to demonstrate glycogen and other polysaccharides. In addition to glycogen, PAS staining can also reveal neutral mucosubstances, certain acidic substances, and structures such as cartilage, pituitary tissue, molds, fungi, pigments, amyloid, and basement membranes. Periodic acid (also known as metaperiodic acid) is a strong oxidizing agent that oxidizes 1,2-glycol groups in carbohydrates and related substances to dialdehydes. These aldehydes then react with Schiff reagent to form a magenta-colored compound. Since periodic acid can also oxidize other intracellular components, it is critical to select appropriate concentrations and oxidation durations to ensure that oxidation is sufficient to convert glycol groups to aldehydes without over-oxidation.
EnkiLife Glycogen PAS Staining Solution features a proprietary formulation that significantly enhances staining performance. It offers high stability and specificity, and is easy to use, requiring only approximately 1 hour.
Basic Information
Product name | Glycogen Periodic Acid-Schiff (PAS) Staining Kit |
Sizes | 50 mL, 100 mL |
Storage | 2-8 ℃，keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Product Components
Components | 4x 50mL | 4x 100mL |
Reagent (A): Periodic Acid Solution | 50 mL | 100 mL |
Reagent (B): Schiff Reagent | 50 mL | 100 mL |
Reagent (C): Hematoxylin Staining Solution | 50 mL | 100 mL |
Reagent (D): Acidic Ethanol Differentiation Solution | 50 mL | 100 mL |
Notes
1. Sections should be thoroughly dewaxed; residual wax will affect staining quality.
2. Do not over-oxidize with periodic acid; optimal temperature is 18–22 °C.
3. Periodic acid solution and Schiff Reagent should be stored at 4 °C in sealed containers. Avoid excessive exposure to air and light. Allow reagents to equilibrate to room temperature for 30 min before use, and perform staining in the dark.
4. Replace acidic ethanol differentiation solution regularly. Differentiation time should be adjusted based on section thickness, tissue type, and freshness of the solution. Ensure adequate rinsing with tap water after differentiation.
5. Exposure time in periodic acid and Schiff Reagent is critical and should be optimized based on section thickness and tissue type.
6. This staining solution is designed for routine tissue sections. For fungi, cells, or extremely thin sections, consider using specialized glycogen PAS staining kits with lower concentrations of periodic acid and hematoxylin to avoid over-staining.
7. Keep staining time short for frozen sections.
8. Use reagents promptly after opening to maintain optimal performance.
