KEY FEATURES
| Full Name | Caspase Activated DNase |
|---|---|
| Synonym | DFF-B; DFFB; CPAN; DFF40; DFF2; DNA Fragmentation Factor 40kDa Beta Polypeptide; Caspase-activated deoxyribonuclease; Caspase-activated nuclease |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CAD in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CAD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CAD. You can calculate the concentration of Human CAD in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CAD ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.54 | 0.95 | 3.62 | 0.5 | 0.97 | 3.67 |
| Standard deviation | 0.03 | 0.06 | 0.12 | 0.03 | 0.05 | 0.13 |
| CV(%) | 6.22 | 5.89 | 3.3 | 5.69 | 5.23 | 3.51 |
RECOVERY
The recovery of Human CAD spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 94 |
| EDTA plasma (n=8) | 90-104 | 95 |
| Cell culture media (n=8) | 85-98 | 91 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CAD in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CAD ELISA Kits
| CASP3 ELISA Kit | publications with CAD and CASP3 |
| DFFA ELISA Kit | publications with CAD and DFFA |
| DNASE2 ELISA Kit | publications with CAD and DNASE2 |
| CIDEA ELISA Kit | publications with CAD and CIDEA |
| CIDEB ELISA Kit | publications with CAD and CIDEB |
Pathways associated with CAD ELISA Kit
| Activation Of DNA Fragmentation Factor Pathway | Activation Of DNA Fragmentation Factor Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
| Apoptosis Modulation And Signaling Pathway | Apoptosis Modulation And Signaling Pathway |
| Apoptosis Induced DNA Fragmentation Pathway | Apoptosis Induced DNA Fragmentation Pathway |
| Apoptotic Execution Phase Pathway | Apoptotic Execution Phase Pathway |
| Caspase Cascade In Apoptosis Pathway | Caspase Cascade In Apoptosis Pathway |
| FAS Pathway And Stress Induction Of HSP Regulation | FAS Pathway And Stress Induction Of HSP Regulation |
Diseases associated with CAD ELISA Kit
| Necrosis | publications with CAD and Necrosis |
| Carcinoma | publications with CAD and Carcinoma |
| Adenocarcinoma | publications with CAD and Adenocarcinoma |
| Immune System Diseases | publications with CAD and Immune System Diseases |
| Neoplasms, Experimental | publications with CAD and Neoplasms, Experimental |
| Cognition Disorders | publications with CAD and Cognition Disorders |
| Cardiovascular Diseases | publications with CAD and Cardiovascular Diseases |
| Disease Models, Animal | publications with CAD and Disease Models, Animal |
| Inflammation | publications with CAD and Inflammation |
| Lung Neoplasms | publications with CAD and Lung Neoplasms |
Organs/Tissues associated with CAD ELISA Kit
| Brain | publications with CAD and Brain |
| Lung | publications with CAD and Lung |
| Liver | publications with CAD and Liver |
| Bone | publications with CAD and Bone |
| Vascular | publications with CAD and Vascular |
| Connective Tissue | publications with CAD and Connective Tissue |
| Kidney | publications with CAD and Kidney |
| Skin | publications with CAD and Skin |
| Testis | publications with CAD and Testis |
| Bone Marrow | publications with CAD and Bone Marrow |
