KEY FEATURES
| Full Name | Cluster Of Differentiation |
|---|---|
| Synonym | M130; MM130; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.62-40ng/mL |
| Sensitivity | 0.28ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CD163 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD163. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD163 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD163, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD163. You can calculate the concentration of Human CD163 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CD163 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.14 | 5.89 | 14.3 | 2.06 | 5.95 | 13.44 |
| Standard deviation | 0.13 | 0.25 | 0.52 | 0.14 | 0.29 | 0.66 |
| CV(%) | 5.95 | 4.21 | 3.61 | 6.87 | 4.93 | 4.91 |
RECOVERY
The recovery of Human CD163 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-100 | 93 |
| EDTA plasma (n=8) | 87-99 | 92 |
| Cell culture media (n=8) | 94-107 | 99 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CD163 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CNTF ELISA Kits
| STAT3 clia kit | publications with CNTF and STAT3 |
| LIFR clia kit | publications with CNTF and LIFR |
| IL6ST clia kit | publications with CNTF and IL6ST |
| CNTFR clia kit | publications with CNTF and CNTFR |
| GFAP clia kit | publications with CNTF and GFAP |
| EGF clia kit | publications with CNTF and EGF |
| SOCS3 clia kit | publications with CNTF and SOCS3 |
| APOE clia kit | publications with CNTF and APOE |
| IL6R clia kit | publications with CNTF and IL6R |
Pathways associated with CNTF ELISA Kit
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Delta-Notch Signaling Pathway | Delta-Notch Signaling Pathway |
| Jak-STAT Signaling Pathway | Jak-STAT Signaling Pathway |
Diseases associated with CNTF ELISA Kit
| Nervous System Diseases | publications with CNTF and Nervous System Diseases |
| Death | publications with CNTF and Death |
| Brain Diseases | publications with CNTF and Brain Diseases |
| Disease Models, Animal | publications with CNTF and Disease Models, Animal |
| Nerve Degeneration | publications with CNTF and Nerve Degeneration |
| Inflammation | publications with CNTF and Inflammation |
| Drug Toxicity | publications with CNTF and Drug Toxicity |
| Necrosis | publications with CNTF and Necrosis |
| Weight Loss | publications with CNTF and Weight Loss |
| Atrophy | publications with CNTF and Atrophy |
Organs/Tissues associated with CNTF ELISA Kit
| Nerve | publications with CNTF and Nerve |
| Brain | publications with CNTF and Brain |
| Eye | publications with CNTF and Eye |
| Embryonic Tissue | publications with CNTF and Embryonic Tissue |
| Ganglia | publications with CNTF and Ganglia |
| Muscle | publications with CNTF and Muscle |
| Spinal Cord | publications with CNTF and Spinal Cord |
| Blood | publications with CNTF and Blood |
| Bone | publications with CNTF and Bone |
| Vascular | publications with CNTF and Vascular |
