KEY FEATURES
| Full Name | FMS Like Tyrosine Kinase 3 |
|---|---|
| Synonym | CD135; STK1; FLK2; Fms-Related Tyrosine Kinase 3; Stem Cell Tyrosine Kinase 1; FL Cytokine Receptor; Fetal Liver Kinase-2 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.13ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human Flt3 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Flt3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Flt3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Flt3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Flt3. You can calculate the concentration of Human Flt3 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human Flt3 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.01 | 1.82 | 8.3 | 1.04 | 1.89 | 8.04 |
| Standard deviation | 0.06 | 0.1 | 0.26 | 0.05 | 0.09 | 0.26 |
| CV(%) | 5.68 | 5.28 | 3.19 | 5.11 | 4.58 | 3.25 |
RECOVERY
The recovery of Human Flt3 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-105 | 97 |
| EDTA plasma (n=8) | 96-110 | 102 |
| Cell culture media (n=8) | 90-102 | 97 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human Flt3 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with SCF ELISA Kits
| Kit ELISA Kit | publications with SCF and Kit |
| Stat3 ELISA Kit | publications with SCF and Stat3 |
Pathways associated with SCF ELISA Kit
| ARMS-mediated Activation Pathway | ARMS-mediated Activation Pathway |
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Axon Guidance Pathway | Axon Guidance Pathway |
| Cytokine Signaling In Immune System Pathway | Cytokine Signaling In Immune System Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| DAP12 Interactions Pathway | DAP12 Interactions Pathway |
| DAP12 Signaling Pathway | DAP12 Signaling Pathway |
| Developmental Biology Pathway | Developmental Biology Pathway |
| Downstream Signal Transduction Pathway | Downstream Signal Transduction Pathway |
Diseases associated with SCF ELISA Kit
| Disease Models, Animal | publications with SCF and Disease Models, Animal |
| Neoplasms, Experimental | publications with SCF and Neoplasms, Experimental |
| Inflammation | publications with SCF and Inflammation |
| Infertility, Male | publications with SCF and Infertility, Male |
| Embryo Loss | publications with SCF and Embryo Loss |
| Cell Transformation, Neoplastic | publications with SCF and Cell Transformation, Neoplastic |
| Fibrosis | publications with SCF and Fibrosis |
| Weight Loss | publications with SCF and Weight Loss |
| Carcinoma | publications with SCF and Carcinoma |
| Skin Neoplasms | publications with SCF and Skin Neoplasms |
Organs/Tissues associated with SCF ELISA Kit
| Bone | publications with SCF and Bone |
| Ovary | publications with SCF and Ovary |
| Skin | publications with SCF and Skin |
| Bone Marrow | publications with SCF and Bone Marrow |
| Testis | publications with SCF and Testis |
| Liver | publications with SCF and Liver |
| Embryonic Tissue | publications with SCF and Embryonic Tissue |
| Heart | publications with SCF and Heart |
| Muscle | publications with SCF and Muscle |
| Spleen | publications with SCF and Spleen |
