KEY FEATURES
| Full Name | Interleukin 5 Receptor Alpha |
|---|---|
| Synonym | CDw125; CD125; IL5R-A; IL5-RA; HSIL5R3; IL5R; IL5R-A |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 78-5000pg/mL |
| Sensitivity | 30pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human IL-5Ra in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-5Ra. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-5Ra and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-5Ra, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-5Ra. You can calculate the concentration of Human IL-5Ra in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human IL-5Ra ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 265.16 | 741 | 2169.31 | 259.66 | 771.56 | 2279.62 |
| Standard deviation | 13.6 | 36.75 | 92.63 | 17.84 | 41.05 | 93.24 |
| CV(%) | 5.13 | 4.96 | 4.27 | 6.87 | 5.32 | 4.09 |
RECOVERY
The recovery of Human IL-5Ra spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-103 | 96 |
| EDTA plasma (n=8) | 96-109 | 102 |
| Cell culture media (n=8) | 86-97 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human IL-5Ra in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with IL5Ra ELISA Kits
| CSF2RB ELISA Kit | publications with IL5Ra and CSF2RB |
| IL5 ELISA Kit | publications with IL5Ra and IL5 |
| JAK2 ELISA Kit | publications with IL5Ra and JAK2 |
| JAK1 ELISA Kit | publications with IL5Ra and JAK1 |
| LYN ELISA Kit | publications with IL5Ra and LYN |
| PTPN11 ELISA Kit | publications with IL5Ra and PTPN11 |
| GRB2 ELISA Kit | publications with IL5Ra and GRB2 |
| SDCBP ELISA Kit | publications with IL5Ra and SDCBP |
Pathways associated with IL5Ra ELISA Kit
| Cytokine Signaling In Immune System Pathway | Cytokine Signaling In Immune System Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Hematopoietic Cell Lineage Pathway | Hematopoietic Cell Lineage Pathway |
| IL-5 Signaling Pathway | IL-5 Signaling Pathway |
| Immune System Pathway | Immune System Pathway |
| Inflammatory Response Pathway | Inflammatory Response Pathway |
| Interleukin Receptor SHC Signaling Pathway | Interleukin Receptor SHC Signaling Pathway |
| Interleukin-2 Signaling Pathway | Interleukin-2 Signaling Pathway |
Diseases associated with IL5Ra ELISA Kit
| Immune System Diseases | publications with IL5Ra and Immune System Diseases |
| Asthma | publications with IL5Ra and Asthma |
| Hypersensitivity | publications with IL5Ra and Hypersensitivity |
| Inflammation | publications with IL5Ra and Inflammation |
| Lung Diseases | publications with IL5Ra and Lung Diseases |
| Necrosis | publications with IL5Ra and Necrosis |
| Bone Marrow Diseases | publications with IL5Ra and Bone Marrow Diseases |
| Brain Diseases | publications with IL5Ra and Brain Diseases |
| Carcinoma | publications with IL5Ra and Carcinoma |
| Neoplasms, Experimental | publications with IL5Ra and Neoplasms, Experimental |
Organs/Tissues associated with IL5Ra ELISA Kit
| Bone | publications with IL5Ra and Bone |
| Lung | publications with IL5Ra and Lung |
| Skin | publications with IL5Ra and Skin |
| Brain | publications with IL5Ra and Brain |
| Trachea | publications with IL5Ra and Trachea |
