KEY FEATURES
| Full Name | Killer Cell Lectin Like Receptor Subfamily C, Member 2 |
|---|---|
| Synonym | CD159c; NKG2C; NKG2-C Type II Integral Membrane Protein; CD159 antigen-like family member C; NK cell receptor C; NKG2-C-activating NK receptor |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human KLRC2 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KLRC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KLRC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KLRC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KLRC2. You can calculate the concentration of Human KLRC2 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human KLRC2 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.54 | 1.2 | 4.5 | 0.56 | 1.3 | 4.47 |
| Standard deviation | 0.03 | 0.05 | 0.23 | 0.04 | 0.07 | 0.24 |
| CV(%) | 5.97 | 4.04 | 5 | 6.68 | 5.4 | 5.47 |
RECOVERY
The recovery of Human KLRC2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma (n=8) | 92-104 | 98 |
| Cell culture media (n=8) | 94-110 | 102 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human KLRC2 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with KLRC2 ELISA Kits
| KLRD1 ELISA Kit | publications with KLRC2 and KLRD1 |
| KLRC3 ELISA Kit | publications with KLRC2 and KLRC3 |
| HLA-C ELISA Kit | publications with KLRC2 and HLA-C |
| TYROBP ELISA Kit | publications with KLRC2 and TYROBP |
Pathways associated with KLRC2 ELISA Kit
| Antigen Processing And Presentation Pathway | Antigen Processing And Presentation Pathway |
| DAP12 Interactions Pathway | DAP12 Interactions Pathway |
| DAP12 Signaling Pathway | DAP12 Signaling Pathway |
| Immune System Pathway | Immune System Pathway |
| Innate Immune System Pathway | Innate Immune System Pathway |
| Natural Killer Cell Mediated Cytotoxicity Pathway | Natural Killer Cell Mediated Cytotoxicity Pathway |
Diseases associated with KLRC2 ELISA Kit
| Liver Diseases | publications with KLRC2 and Liver Diseases |
| Inflammation | publications with KLRC2 and Inflammation |
| Carcinoma | publications with KLRC2 and Carcinoma |
| Demyelinating Diseases | publications with KLRC2 and Demyelinating Diseases |
| Cardiovascular Diseases | publications with KLRC2 and Cardiovascular Diseases |
| Liver Neoplasms | publications with KLRC2 and Liver Neoplasms |
| Adenocarcinoma | publications with KLRC2 and Adenocarcinoma |
| Encephalomyelitis, Autoimmune, Experimental | publications with KLRC2 and Encephalomyelitis, Autoimmune, Experimental |
| Drug-Induced Liver Injury | publications with KLRC2 and Drug-Induced Liver Injury |
| Kidney Diseases | publications with KLRC2 and Kidney Diseases |
Organs/Tissues associated with KLRC2 ELISA Kit
| Blood | publications with KLRC2 and Blood |
| Liver | publications with KLRC2 and Liver |
| Brain | publications with KLRC2 and Brain |
| Cervix | publications with KLRC2 and Cervix |
