KEY FEATURES
| Full Name | Lymphotoxin Beta |
|---|---|
| Synonym | LT-B; p33; TNFC; TNF-C; TNFSF3; Tumor necrosis factor ligand superfamily member 3; Tumor Necrosis Factor C |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human LTb in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LTb. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LTb and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LTb, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LTb. You can calculate the concentration of Human LTb in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human LTb ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 46.99 | 154.31 | 348.88 | 49.32 | 167.31 | 358.26 |
| Standard deviation | 2.94 | 8.22 | 18.07 | 2.86 | 9.07 | 14.22 |
| CV(%) | 6.25 | 5.33 | 5.18 | 5.79 | 5.42 | 3.97 |
RECOVERY
The recovery of Human LTb spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-103 | 95 |
| EDTA plasma (n=8) | 88-102 | 94 |
| Cell culture media (n=8) | 86-97 | 91 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human LTb in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with LTB ELISA Kits
| LTBR ELISA Kit | publications with LTB and LTBR |
| TNFSF14 ELISA Kit | publications with LTB and TNFSF14 |
| RELB ELISA Kit | publications with LTB and RELB |
| NFKB2 ELISA Kit | publications with LTB and NFKB2 |
| AIRE ELISA Kit | publications with LTB and AIRE |
| LST1 ELISA Kit | publications with LTB and LST1 |
| SOSTDC1 ELISA Kit | publications with LTB and SOSTDC1 |
Pathways associated with LTB ELISA Kit
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| NF-kappa B Signaling Pathway | NF-kappa B Signaling Pathway |
| Rheumatoid Arthritis Pathway | Rheumatoid Arthritis Pathway |
| Spinal Cord Injury Pathway | Spinal Cord Injury Pathway |
Diseases associated with LTB ELISA Kit
| Inflammation | publications with LTB and Inflammation |
| Necrosis | publications with LTB and Necrosis |
| Lung Diseases | publications with LTB and Lung Diseases |
| Neoplasms | publications with LTB and Neoplasms |
| Cardiovascular Diseases | publications with LTB and Cardiovascular Diseases |
| Edema | publications with LTB and Edema |
| Drug Toxicity | publications with LTB and Drug Toxicity |
| Atherosclerosis | publications with LTB and Atherosclerosis |
| Colitis | publications with LTB and Colitis |
| Brain Diseases | publications with LTB and Brain Diseases |
Organs/Tissues associated with LTB ELISA Kit
| Blood | publications with LTB and Blood |
| Spleen | publications with LTB and Spleen |
| Lymph Node | publications with LTB and Lymph Node |
| Lymph | publications with LTB and Lymph |
| Intestine | publications with LTB and Intestine |
| Bone Marrow | publications with LTB and Bone Marrow |
| Brain | publications with LTB and Brain |
| Embryonic Tissue | publications with LTB and Embryonic Tissue |
| Ovary | publications with LTB and Ovary |
| Connective Tissue | publications with LTB and Connective Tissue |
