KEY FEATURES
| Full Name | Macrophage Inflammatory Protein 1 Alpha |
|---|---|
| Synonym | CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6.3pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human MIP1a in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIP1a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP1a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP1a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIP1a. You can calculate the concentration of Human MIP1a in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human MIP1a ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 52.05 | 145.68 | 399.4 | 47.91 | 133.49 | 384.51 |
| Standard deviation | 3.48 | 6.24 | 17.77 | 3.27 | 7.56 | 15.92 |
| CV(%) | 6.68 | 4.28 | 4.45 | 6.82 | 5.66 | 4.14 |
RECOVERY
The recovery of Human MIP1a spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-101 | 94 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 95-108 | 100 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human MIP1a in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CCL3 ELISA Kits
| CCL4 ELISA Kit | publications with CCL3 and CCL4 |
| CCR5 ELISA Kit | publications with CCL3 and CCR5 |
| CCR1 ELISA Kit | publications with CCL3 and CCR1 |
| CCR2 ELISA Kit | publications with CCL3 and CCR2 |
| CCR3 ELISA Kit | publications with CCL3 and CCR3 |
| CCR4 ELISA Kit | publications with CCL3 and CCR4 |
| IL18 ELISA Kit | publications with CCL3 and IL18 |
| IL1B ELISA Kit | publications with CCL3 and IL1B |
| IDE ELISA Kit | publications with CCL3 and IDE |
| CXCR5 ELISA Kit | publications with CCL3 and CXCR5 |
Pathways associated with CCL3 ELISA Kit
| Chagas Disease (American Trypanosomiasis) Pathway | Chagas Disease (American Trypanosomiasis) Pathway |
| Chemokine Receptors Bind Chemokines Pathway | Chemokine Receptors Bind Chemokines Pathway |
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway | Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway |
| Disease Pathway | Disease Pathway |
Diseases associated with CCL3 ELISA Kit
| Inflammation | publications with CCL3 and Inflammation |
| Necrosis | publications with CCL3 and Necrosis |
| Disease Models, Animal | publications with CCL3 and Disease Models, Animal |
| Lung Diseases | publications with CCL3 and Lung Diseases |
| HIV Infections | publications with CCL3 and HIV Infections |
| Cardiovascular Diseases | publications with CCL3 and Cardiovascular Diseases |
| Hypersensitivity | publications with CCL3 and Hypersensitivity |
| Liver Diseases | publications with CCL3 and Liver Diseases |
| Fibrosis | publications with CCL3 and Fibrosis |
| Pneumonia | publications with CCL3 and Pneumonia |
Organs/Tissues associated with CCL3 ELISA Kit
| Blood | publications with CCL3 and Blood |
| Lung | publications with CCL3 and Lung |
| Brain | publications with CCL3 and Brain |
| Liver | publications with CCL3 and Liver |
| Kidney | publications with CCL3 and Kidney |
| Heart | publications with CCL3 and Heart |
| Intestine | publications with CCL3 and Intestine |
| Eye | publications with CCL3 and Eye |
| Uterus | publications with CCL3 and Uterus |
| Pancreas | publications with CCL3 and Pancreas |
