KEY FEATURES
| Full Name | Macrophage Inflammatory Protein 1 Beta |
|---|---|
| Synonym | CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 5.6pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human MIP1b in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIP1b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP1b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP1b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIP1b. You can calculate the concentration of Human MIP1b in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human MIP1b ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 52.93 | 98.48 | 360.24 | 50.39 | 95.11 | 360.16 |
| Standard deviation | 3.69 | 4.17 | 16.97 | 3.32 | 5.17 | 15.7 |
| CV(%) | 6.97 | 4.23 | 4.71 | 6.58 | 5.44 | 4.36 |
RECOVERY
The recovery of Human MIP1b spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-103 | 96 |
| EDTA plasma (n=8) | 84-97 | 90 |
| Cell culture media (n=8) | 94-109 | 100 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human MIP1b in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CCL4 ELISA Kits
| CCL3 ELISA Kit | publications with CCL4 and CCL3 |
| CCR5 ELISA Kit | publications with CCL4 and CCR5 |
| CCR1 ELISA Kit | publications with CCL4 and CCR1 |
| CXCL2 ELISA Kit | publications with CCL4 and CXCL2 |
| CCR2 ELISA Kit | publications with CCL4 and CCR2 |
| CXCR3 ELISA Kit | publications with CCL4 and CXCR3 |
| CCR3 ELISA Kit | publications with CCL4 and CCR3 |
| CCR7 ELISA Kit | publications with CCL4 and CCR7 |
| CCR8 ELISA Kit | publications with CCL4 and CCR8 |
| IL1B ELISA Kit | publications with CCL4 and IL1B |
Pathways associated with CCL4 ELISA Kit
| Chemokine Receptors Bind Chemokines Pathway | Chemokine Receptors Bind Chemokines Pathway |
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Cytosolic DNA-sensing Pathway | Cytosolic DNA-sensing Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| IL12-mediated Signaling Events Pathway | IL12-mediated Signaling Events Pathway |
Diseases associated with CCL4 ELISA Kit
| Immune System Diseases | publications with CCL4 and Immune System Diseases |
| Protein Deficiency | publications with CCL4 and Protein Deficiency |
| Bone Diseases | publications with CCL4 and Bone Diseases |
| Hypertension, Portal | publications with CCL4 and Hypertension, Portal |
| Disease Susceptibility | publications with CCL4 and Disease Susceptibility |
| Gastrointestinal Diseases | publications with CCL4 and Gastrointestinal Diseases |
| Eye Diseases | publications with CCL4 and Eye Diseases |
| Asthma | publications with CCL4 and Asthma |
| Pneumonia | publications with CCL4 and Pneumonia |
| Ischemia | publications with CCL4 and Ischemia |
Organs/Tissues associated with CCL4 ELISA Kit
| Liver | publications with CCL4 and Liver |
| Blood | publications with CCL4 and Blood |
| Lung | publications with CCL4 and Lung |
| Bone Marrow | publications with CCL4 and Bone Marrow |
| Kidney | publications with CCL4 and Kidney |
| Intestine | publications with CCL4 and Intestine |
| Lymph | publications with CCL4 and Lymph |
| Parathyroid | publications with CCL4 and Parathyroid |
| Prostate | publications with CCL4 and Prostate |
| Adrenal Gland | publications with CCL4 and Adrenal Gland |
