KEY FEATURES
| Full Name | Nestin |
|---|---|
| Synonym | NES |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human NES in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NES. You can calculate the concentration of Human NES in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human NES ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.48 | 1.39 | 4.54 | 0.5 | 1.3 | 4.99 |
| Standard deviation | 0.03 | 0.08 | 0.23 | 0.03 | 0.07 | 0.24 |
| CV(%) | 6.02 | 5.62 | 5.06 | 5.11 | 5.41 | 4.8 |
RECOVERY
The recovery of Human NES spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-105 | 96 |
| EDTA plasma (n=8) | 86-101 | 93 |
| Cell culture media (n=8) | 87-100 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human NES in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NES ELISA Kits
| EGF ELISA Kit | publications with NES and EGF |
| BMP4 ELISA Kit | publications with NES and BMP4 |
| MYCN ELISA Kit | publications with NES and MYCN |
| SOX10 ELISA Kit | publications with NES and SOX10 |
| CDK5 ELISA Kit | publications with NES and CDK5 |
| VSX2 ELISA Kit | publications with NES and VSX2 |
| CDK5R1 ELISA Kit | publications with NES and CDK5R1 |
Diseases associated with NES ELISA Kit
| Nervous System Diseases | publications with NES and Nervous System Diseases |
| Brain Diseases | publications with NES and Brain Diseases |
| Disease Models, Animal | publications with NES and Disease Models, Animal |
| Cardiovascular Diseases | publications with NES and Cardiovascular Diseases |
| Seizures | publications with NES and Seizures |
| Nerve Degeneration | publications with NES and Nerve Degeneration |
| Breast Neoplasms | publications with NES and Breast Neoplasms |
| Adenocarcinoma | publications with NES and Adenocarcinoma |
| Heart Diseases | publications with NES and Heart Diseases |
| Necrosis | publications with NES and Necrosis |
Organs/Tissues associated with NES ELISA Kit
| Nerve | publications with NES and Nerve |
| Brain | publications with NES and Brain |
| Embryonic Tissue | publications with NES and Embryonic Tissue |
| Muscle | publications with NES and Muscle |
| Vascular | publications with NES and Vascular |
| Eye | publications with NES and Eye |
| Skin | publications with NES and Skin |
| Pancreas | publications with NES and Pancreas |
| Heart | publications with NES and Heart |
| Kidney | publications with NES and Kidney |
