KEY FEATURES
| Full Name | Transcription Factor P65 |
|---|---|
| Synonym | Nuclear factor NF-kappa-B p65 subunit; NFKBP65; RELA; p65; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human NFKB3 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NFKB3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFKB3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFKB3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NFKB3. You can calculate the concentration of Human NFKB3 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human NFKB3 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.47 | 1.54 | 4.14 | 0.45 | 1.66 | 3.82 |
| Standard deviation | 0.03 | 0.07 | 0.21 | 0.03 | 0.09 | 0.16 |
| CV(%) | 5.49 | 4.43 | 5.14 | 5.73 | 5.45 | 4.19 |
RECOVERY
The recovery of Human NFKB3 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-102 | 95 |
| EDTA plasma (n=8) | 89-102 | 96 |
| Cell culture media (n=8) | 89-99 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human NFKB3 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with RELA ELISA Kits
| REL ELISA Kit | publications with RELA and REL |
| JUN ELISA Kit | publications with RELA and JUN |
| IKBKB ELISA Kit | publications with RELA and IKBKB |
| RELB ELISA Kit | publications with RELA and RELB |
| CHUK ELISA Kit | publications with RELA and CHUK |
| NFKB1 ELISA Kit | publications with RELA and NFKB1 |
| NFKB2 ELISA Kit | publications with RELA and NFKB2 |
| CCND1 ELISA Kit | publications with RELA and CCND1 |
| SP1 ELISA Kit | publications with RELA and SP1 |
| MYC ELISA Kit | publications with RELA and MYC |
Pathways associated with RELA ELISA Kit
| AGE/RAGE Pathway | AGE/RAGE Pathway |
| Activated TLR4 Signalling Pathway | Activated TLR4 Signalling Pathway |
| Activation Of NF-kappaB In B Cells Pathway | Activation Of NF-kappaB In B Cells Pathway |
| Acute Myeloid Leukemia Pathway | Acute Myeloid Leukemia Pathway |
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Adipocytokine Signaling Pathway | Adipocytokine Signaling Pathway |
| Amoebiasis Pathway | Amoebiasis Pathway |
Diseases associated with RELA ELISA Kit
| Necrosis | publications with RELA and Necrosis |
| Inflammation | publications with RELA and Inflammation |
| Neoplasms | publications with RELA and Neoplasms |
| Liver Diseases | publications with RELA and Liver Diseases |
| Nervous System Diseases | publications with RELA and Nervous System Diseases |
| Drug Toxicity | publications with RELA and Drug Toxicity |
| Brain Diseases | publications with RELA and Brain Diseases |
| Kidney Diseases | publications with RELA and Kidney Diseases |
| Breast Neoplasms | publications with RELA and Breast Neoplasms |
| Neoplasms, Experimental | publications with RELA and Neoplasms, Experimental |
Organs/Tissues associated with RELA ELISA Kit
| Blood | publications with RELA and Blood |
| Liver | publications with RELA and Liver |
| Vascular | publications with RELA and Vascular |
| Lung | publications with RELA and Lung |
| Muscle | publications with RELA and Muscle |
| Brain | publications with RELA and Brain |
| Bone | publications with RELA and Bone |
| Kidney | publications with RELA and Kidney |
| Intestine | publications with RELA and Intestine |
| Heart | publications with RELA and Heart |
