KEY FEATURES
| Full Name | Neurotensin |
|---|---|
| Synonym | NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N |
| Assay Type | Competitive |
| Reactivity | Human |
| Range | 37-3000pg/mL |
| Sensitivity | 13.2pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 50μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human NT in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human NT. During the reaction, Human NT in the sample or standard competes with a fixed amount of Human NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human NT in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human NT ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 166.04 | 336.59 | 1357.11 | 150.84 | 344.44 | 1475.95 |
| Standard deviation | 10.41 | 19.15 | 51.43 | 8.57 | 17.98 | 61.99 |
| CV(%) | 6.27 | 5.69 | 3.79 | 5.68 | 5.22 | 4.2 |
RECOVERY
The recovery of Human NT spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-104 | 96 |
| EDTA plasma (n=8) | 93-106 | 100 |
| Cell culture media (n=8) | 95-110 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human NT in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NT ELISA Kits
| CCK ELISA Kit | publications with NT and CCK |
| NTSR1 ELISA Kit | publications with NT and NTSR1 |
| NTSR2 ELISA Kit | publications with NT and NTSR2 |
| GRP ELISA Kit | publications with NT and GRP |
| TAC1 ELISA Kit | publications with NT and TAC1 |
| NMU ELISA Kit | publications with NT and NMU |
Pathways associated with NT ELISA Kit
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| G Alpha (q) Signalling Events Pathway | G Alpha (q) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK | Gastrin-CREB Signalling Pathway Via PKC And MAPK |
| Peptide Ligand-binding Receptors Pathway | Peptide Ligand-binding Receptors Pathway |
| Signal Transduction Pathway | Signal Transduction Pathway |
| Signaling By GPCR Pathway | Signaling By GPCR Pathway |
Diseases associated with NT ELISA Kit
| Cardiovascular Diseases | publications with NT and Cardiovascular Diseases |
| Hypertension | publications with NT and Hypertension |
| Nervous System Diseases | publications with NT and Nervous System Diseases |
| Bradycardia | publications with NT and Bradycardia |
| Hypotension | publications with NT and Hypotension |
| Inflammation | publications with NT and Inflammation |
| Brain Diseases | publications with NT and Brain Diseases |
| Disease Models, Animal | publications with NT and Disease Models, Animal |
| Kidney Diseases | publications with NT and Kidney Diseases |
| Pain | publications with NT and Pain |
Organs/Tissues associated with NT ELISA Kit
| Brain | publications with NT and Brain |
| Heart | publications with NT and Heart |
| Kidney | publications with NT and Kidney |
| Stomach | publications with NT and Stomach |
| Intestine | publications with NT and Intestine |
| Skin | publications with NT and Skin |
| Mouth | publications with NT and Mouth |
| Connective Tissue | publications with NT and Connective Tissue |
| Pancreas | publications with NT and Pancreas |
| Ovary | publications with NT and Ovary |
