Product Introduction
Mast cells are commonly found in loose connective tissue, often distributed in groups along small blood vessels and lymphatics, and are also frequently observed around interlobular ducts in the bronchi and pancreas. These cells are generally large, about 20–30 μm in diameter, round or oval in shape, with a small nucleus and cytoplasm filled with coarse, metachromatic, eosinophilic granules.
The staining mechanism of EnkiLife Mast Cell Staining Solution (Aldehyde Fuchsin-Orange G Method) is based on the strong affinity of aldehyde fuchsin for acidic mucopolysaccharides containing sulfate groups. Mast cell granules contain heparin with carboxyl and sulfate groups, which readily combine with aldehyde fuchsin to form a colored complex. Orange G is an acidic dye that stains other tissue components yellow. This method can demonstrate metachromatic granules. After staining, mast cell granules appear deep purple, other cells are mostly unstained, and the background appears orange-yellow, providing a sharp contrast.
Basic Information
Product name | Mast Cell Staining Kit, Aldehyde Fuchsin-Orange G Method |
Sizes | 4x 20 mL |
Storage | 2-8℃，keep away from light |
Shipping | Shipped with ice pack |
Validity | 6 months |
Product Components
Components | 4x 20mL |
Reagent (A): Weigert's Iodine Solution | 20 mL |
Reagent (B): Weigert's Differentiation Solution | 50 mL |
Reagent (C): Aldehyde Fuchsin Staining Solution | 20 mL |
Reagent (D): Orange G Staining Solution | 20 mL |
Notes
1. For mast cell staining, tissue should be fresh and fixed immediately after removal.
2. If 10% neutral formalin fixative is not available, 4% formaldehyde in physiological saline may be used as a substitute.
3. Control the staining intensity of Orange G to avoid over-staining, which may obscure the purple granules.
4. After differentiation, quickly proceed to 95% ethanol, absolute ethanol, and xylene. Prolonged exposure may cause fading.
5. Use reagents promptly after opening to avoid affecting experimental results.
6. For safety and health, wear lab coats and disposable gloves during operation.
