Product Introduction
Cartilage tissue is composed of chondrocytes and cartilage matrix. Cartilage tissue and its surrounding perichondrium constitute cartilage. Based on the fiber components in the matrix, cartilage is classified into hyaline cartilage, elastic cartilage, and fibrocartilage. There are various cartilage staining methods, such as toluidine blue, Alcian blue, and Safranin O methods.
The staining principle of EnkiLife Modified Safranin O-Fast Green Cartilage Staining Method is that basophilic cartilage binds to the basic dye Safranin O, appearing red, while acidic bone tissue binds to the acidic dye Fast Green, appearing green or blue. The contrast between red cartilage and green/blue bone tissue allows clear differentiation. Safranin O is a cationic dye that binds to polyanions. It stains cartilage by binding to anionic groups (e.g., chondroitin sulfate or keratan sulfate) in proteoglycans. The intensity of Safranin O staining is approximately proportional to the concentration of anions, indirectly reflecting the content and distribution of proteoglycans in the matrix. When cartilage is damaged, glycoproteins are released, leading to uneven matrix composition, resulting in weak or no staining by Safranin O. Quantitative analysis of Safranin O-stained cartilage matrix can be performed using image analysis software. Fast Green binds to collagen fibers and is resistant to fading. Differentiation is critical in Safranin O-Fast Green staining: over-differentiation may lead to no staining, while under-differentiation may result in overly intense staining.
Basic Information
Product name | Modified Safranin O-Fast Green Cartilage Staining Kit |
Sizes | 50 mL, 100 mL |
Storage | RT |
Shipping | RT |
Validity | 12 months |
Product Components
Components | 5x 50mL | 5x 100mL | |
Reagent (A): | A1: Weigert Solution A | 25 mL | 50 mL |
A2: Weigert Solution B | 25 mL | 50 mL | |
Mix A1 and A2 in equal parts before use. Do not prepare in advance. Weigert stain loses activity after 24 h. | |||
Reagent (B): Acid Ethanol Differentiation Solution | 50 mL | 100 mL | |
Reagent (C): Fast Green Staining Solution | 50 mL | 100 mL | |
Reagent (D): Acetic Acid Solution | 50 mL | 100 mL | |
Reagent (E): Safranin O Staining Solution | 50 mL | 100 mL | |
Notes
1. To visualize nuclei, iron hematoxylin is recommended due to its strong staining intensity and deep tone. Ordinary hematoxylin may not stain strongly enough.
2. Weigert stain should not be prepared in advance; it loses staining activity after 24 h.
3. If large volumes of acetic acid solution are needed, prepare 0.05–0.1% acetic acid aqueous solution as substitute.
4. Do not overstain with Safranin O, as it may mix with green and produce a purple-blue color.
5. Do not arbitrarily extend staining time after Safranin O application.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
