KEY FEATURES
| Full Name | Complement Component 3a |
|---|---|
| Synonym | C3a-desArg;ASP; |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.16-10ng/mL |
| Sensitivity | 0.1ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse C3a in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3a. You can calculate the concentration of Mouse C3a in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse C3a ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.48 | 1.44 | 3.63 | 0.46 | 1.43 | 3.41 |
| Standard deviation | 0.03 | 0.06 | 0.19 | 0.03 | 0.07 | 0.18 |
| CV(%) | 6.99 | 4.16 | 5.27 | 5.7 | 5.17 | 5.2 |
RECOVERY
The recovery of Mouse C3a spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma (n=8) | 86-99 | 91 |
| Cell culture media (n=8) | 90-104 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse C3a in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with C3 ELISA Kits
| CR1 ELISA Kit | publications with C3 and CR1 |
| CR2 ELISA Kit | publications with C3 and CR2 |
| CD46 ELISA Kit | publications with C3 and CD46 |
| CFH ELISA Kit | publications with C3 and CFH |
| ITGAM ELISA Kit | publications with C3 and ITGAM |
| CFI ELISA Kit | publications with C3 and CFI |
| ITGAX ELISA Kit | publications with C3 and ITGAX |
| C3AR1 ELISA Kit | publications with C3 and C3AR1 |
| CFD ELISA Kit | publications with C3 and CFD |
Pathways associated with C3 ELISA Kit
| Activation Of C3 And C5 Pathway | Activation Of C3 And C5 Pathway |
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Alternative Complement Activation Pathway | Alternative Complement Activation Pathway |
| Chagas Disease (American Trypanosomiasis) Pathway | Chagas Disease (American Trypanosomiasis) Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Complement Activation, Classical Pathway | Complement Activation, Classical Pathway |
| Complement And Coagulation Cascades Pathway | Complement And Coagulation Cascades Pathway |
Diseases associated with C3 ELISA Kit
| Kidney Diseases | publications with C3 and Kidney Diseases |
| Neoplasms | publications with C3 and Neoplasms |
| Nervous System Diseases | publications with C3 and Nervous System Diseases |
| Cardiovascular Diseases | publications with C3 and Cardiovascular Diseases |
| Inflammation | publications with C3 and Inflammation |
| Proteinuria | publications with C3 and Proteinuria |
| Lung Diseases | publications with C3 and Lung Diseases |
| Liver Diseases | publications with C3 and Liver Diseases |
| Pain | publications with C3 and Pain |
| Necrosis | publications with C3 and Necrosis |
Organs/Tissues associated with C3 ELISA Kit
| Blood | publications with C3 and Blood |
| Kidney | publications with C3 and Kidney |
| Bone | publications with C3 and Bone |
| Skin | publications with C3 and Skin |
| Brain | publications with C3 and Brain |
| Liver | publications with C3 and Liver |
| Muscle | publications with C3 and Muscle |
| Spinal Cord | publications with C3 and Spinal Cord |
| Nerve | publications with C3 and Nerve |
| Lung | publications with C3 and Lung |
