KEY FEATURES
| Full Name | Alpha-1-Microglobulin |
|---|---|
| Synonym | AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin |
| Assay Type | Competitive |
| Reactivity | Mouse |
| Range | 2.47-200μg/mL |
| Sensitivity | 0.94μg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 50μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse a1M in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse a1M. During the reaction, Mouse a1M in the sample or standard competes with a fixed amount of Mouse a1M on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse a1M. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse a1M in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse a1M ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (μg/mL) | 9.36 | 24.55 | 84.76 | 9.48 | 23.72 | 91.84 |
| Standard deviation | 0.61 | 1.07 | 3.27 | 0.64 | 1.42 | 2.83 |
| CV(%) | 6.48 | 4.34 | 3.86 | 6.74 | 5.97 | 3.08 |
RECOVERY
The recovery of Mouse a1M spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 96 |
| EDTA plasma (n=8) | 86-99 | 93 |
| Cell culture media (n=8) | 95-109 | 102 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse a1M in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with a1M ELISA Kits
| SERPINC1 ELISA Kit | publications with a1M and SERPINC1 |
| AHSG ELISA Kit | publications with a1M and AHSG |
| ALB ELISA Kit | publications with a1M and ALB |
| APOA1 ELISA Kit | publications with a1M and APOA1 |
| ITIH3 ELISA Kit | publications with a1M and ITIH3 |
| ITIH2 ELISA Kit | publications with a1M and ITIH2 |
Pathways associated with a1M ELISA Kit
| Binding And Uptake Of Ligands By Scavenger Receptors Pathway | Binding And Uptake Of Ligands By Scavenger Receptors Pathway |
| Scavenging Of Heme From Plasma Pathway | Scavenging Of Heme From Plasma Pathway |
Diseases associated with a1M ELISA Kit
| Hypertension | publications with a1M and Hypertension |
| Inflammation | publications with a1M and Inflammation |
| Necrosis | publications with a1M and Necrosis |
| Kidney Diseases | publications with a1M and Kidney Diseases |
| Hemorrhage | publications with a1M and Hemorrhage |
| Neoplasms | publications with a1M and Neoplasms |
| Liver Diseases | publications with a1M and Liver Diseases |
| Brain Diseases | publications with a1M and Brain Diseases |
| Drug-Induced Liver Injury | publications with a1M and Drug-Induced Liver Injury |
| Disease Models, Animal | publications with a1M and Disease Models, Animal |
Organs/Tissues associated with a1M ELISA Kit
| Heart | publications with a1M and Heart |
| Liver | publications with a1M and Liver |
| Kidney | publications with a1M and Kidney |
| Intestine | publications with a1M and Intestine |
| Brain | publications with a1M and Brain |
| Muscle | publications with a1M and Muscle |
| Lung | publications with a1M and Lung |
| Pancreas | publications with a1M and Pancreas |
| Embryonic Tissue | publications with a1M and Embryonic Tissue |
| Spleen | publications with a1M and Spleen |
