Use at an assay-dependent concentration. For Sandwich ELISA pairing: Mouse IL-17E/IL-25 Antibody(Detector) (EPA1780D), with Mouse IL-17E/IL-25 Protein (EPA1780S) as assay standards. The reference range value is 78.1-10,000 pg/mL.
Target info
Function
Interleukin-25 (IL-25, also known as IL-17E) is a type-2-inducing member of the IL-17 family encoded in mice by the Il25 (Il17e) gene on chromosome 1. It is a secreted ~18-kDa cystine-knot glycoprotein produced by intestinal tuft cells, mucosal epithelial cells, alveolar epithelial cells, eosinophils, Th2 cells, mast cells, and alveolar macrophages, particularly after helminth infection or exposure to luminal metabolites such as succinate. IL-25 signals through a heterodimeric receptor composed of IL-17RA paired with the private IL-17RB (IL-17BR) chain, expressed on group 2 innate lymphoid cells (ILC2s), Th2 cells, eosinophils, basophils, mast cells, and invariant NKT cells. The receptor cytoplasmic SEFIR domains recruit the adaptor Act1 (TRAF3IP2), which signals through TRAF4/TRAF6 to activate NF-kappa-B, p38 MAPK, JAK2/STAT5A, and GATA3. The principal biological effect is rapid expansion and activation of ILC2s, which secrete large amounts of IL-4, IL-5, IL-9, and IL-13. This drives type 2 effector programs: eosinophilia, goblet-cell mucus secretion, alternative macrophage activation, intestinal tuft-cell hyperplasia during anti-helminth immunity, and allergic airway inflammation. IL-25 also inhibits Th17 differentiation via induction of type 2 cytokines. Of note, tuft cells themselves constitutively express IL-17RB in an autocrine negative-feedback loop that limits IL-25 bioavailability. The cytokine is secreted into the mucosal extracellular space and circulation; no membrane-bound isoform of IL-25 itself is described.
