Product Introduction
The myelin sheath is a membrane that wraps around the axons of nerve cells. It is composed of myelin-forming cells and their cell membranes, forming a multilayered lipid bilayer structure through spiral wrapping of the neural membrane cell’s plasma membrane along the axonal axis. The myelin sheath contains nodes of Ranvier, which allow for saltatory conduction of nerve impulses. Myelin staining is of certain significance in pathological diagnosis. Pathological changes in myelin can be divided into early, middle, and late stages. In the early stage, myelin stains deeply; in the middle stage, degenerated myelin forms lipid droplets, which can be demonstrated with lipid stains; in the late stage, myelin is completely degraded and removed by phagocytes, resulting in loss of positive staining.
Many diseases can cause changes in myelin. Luxol Fast Blue myelin staining can reveal whether the myelin is intact, degenerated, or necrotic under pathological conditions, as well as the extent of repair. It is valuable for pathological diagnosis and research of neural tissue. For example, when nerve fibers are damaged, the myelin may swell, become tortuous and spherical, break, or completely disappear.
Basic Information
Product name | Myelin Staining Kit, Luxol Fast Blue (LFB) Method |
Sizes | 50 mL |
Storage | RT |
Shipping | RT |
Validity | 12 months |
Product Components
Components | 3x 50mL |
Reagent (A): Luxol Fast Blue Staining Solution | 50 mL |
Reagent (B): Luxol differentiation solution | 50 mL |
Reagent (C): Eosin Staining Solution | 50 mL |
Notes
1. The differentiation step is critical. Differentiation time should be strictly controlled and observed under the microscope.
2. Calcium-formalin fixative is preferred; 10% neutral formalin or formalin may also be used.
3. Sections should not be too thick (5–10 μm max) to avoid detachment or over-staining.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Use reagents promptly after opening to maintain optimal performance.
