Product Introduction
Cytological routine staining commonly uses the Papanicolaou (Pap) method. Initially, Papanicolaou staining was only used to assess vaginal epithelial estrogen levels and detect pathogens such as Candida and Trichomonas in the reproductive tract. When Orange G6 is used in combination with EA36 or EA50, the cytoplasm can be stained in distinct colors such as green, blue, and pink. Currently, most laboratories use ready-made staining solutions, so the optimal conditions for each modified solution should be noted. The final cytoplasmic staining should be transparent and visible, and nuclear chromatin should be easily distinguishable. The improved Papanicolaou staining solution contains multiple ions and exhibits polychromatic staining properties. After staining, the cytoplasm appears bright and transparent, and the nuclear membrane, nucleoli, and chromatin structure are clearly visible. The nuclear staining solution is mainly Harris hematoxylin, and the cytoplasmic staining solutions are mainly EA36 and EA50. Papanicolaou staining is used for exfoliated cell specimens: the nucleus appears blue or black, and the cytoplasm of keratinized squamous cells appears pink or orange-red.
EnkiLife Papanicolaou staining solution uses EA50 for cytoplasmic staining and a non-toxic modified hematoxylin solution for nuclear staining. It is suitable not only for gynecological cytology smears such as cervical cancer and precancerous lesion screening, but also for non-gynecological samples such as pleural effusion, ascites, and sputum.
Basic Information
Product name | Papanicolaou (Pap) Staining Kit |
Sizes | 100 mL, 250 mL, 500 mL |
Storage | RT |
Shipping | RT |
Validity | 12 months |
Product Components
Components | 4x 100mL | 4x 250mL | 4x 500mL |
Reagent (A): Hematoxylin Staining Solution | 100 mL | 250 mL | 500 mL |
Reagent (B): Acidic Ethanol Differentiation Solution | 100 mL | 250 mL | 500 mL |
Reagent (C): Orange G6 Staining Solution | 100 mL | 250 mL | 500 mL |
Reagent (D): EA50 Staining Solution | 100 mL | 250 mL | 500 mL |
Notes
1. This reagent has been filtered. If staining results are unsatisfactory, filter again. Replace staining solution regularly.
2. For safety and health, wear lab coats and disposable gloves during operation.
