Product Introduction
Collagen fibers are the most widely distributed and abundant fibers in connective tissue, found extensively in various organs, especially in the skin, sclera, and tendons. Type I collagen is mainly found in bone, skin, and tendon fibers. Type II collagen is primarily located in cartilage. Type III collagen is mainly present in embryonic tissues and adult blood vessels and gastrointestinal tract. Type IV collagen is primarily found in the basement membrane. Both Picrosirius Red and its counterstain are strongly acidic dyes that bind firmly to basic groups in collagen molecules. Under polarized light microscopy, collagen fibers exhibit positive uniaxial birefringence. When combined with Picrosirius Red, this birefringence is enhanced, improving resolution and allowing differentiation between collagen types.
EnkiLife Picrosirius Red Staining Kit consists of Hematoxylin Staining Solution and Picrosirius Red Staining Solution. It is mainly used to study abnormal collagen fibers or fibrosis in various pathological tissues. Under a standard light microscope, collagen fibers in tissues such as heart and blood vessels are stained red. Under polarized light microscopy, it aids in the classification and grading of fibrotic lesions. Although immunohistochemistry can also be used to detect Type I and Type III collagen fibers, the antibodies are expensive and the procedure is time-consuming. In contrast, Picrosirius Red staining is cost-effective and simple to perform.
Basic Information
Product name | Picrosirius Red Staining Kit |
Sizes | 50 mL |
Storage | 2-8 ℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Product Components
Components | 2x 50mL |
Reagent (A): Hematoxylin Staining Solution | 50 mL |
Reagent (B): Picrosirius Red Staining Solution | 50 mL |
Notes
1. For optimal visualization under polarized light, section thickness should be 6–7 μm.
2. Omitting hematoxylin staining will result in light red nuclei and improved contrast between collagen and muscle fibers.
3. Avoid prolonged treatment in low-concentration ethanol after Picrosirius Red staining, as the yellow color of muscle fibers may fade.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
