PVDF Membrane
Special for Western Blotting Experiments · High Loading Capacity · Tear-Resistant
Product Overview
PVDF (Polyvinylidene Fluoride) membrane is a commonly used solid support in Western blotting experiments. It is also suitable for adsorption analysis, amino acid analysis, N-terminal protein sequencing, Dot and Slot blot detection, glycoprotein staining, and lipopolysaccharide analysis.
PVDF membranes are available in two main pore sizes: 0.22μm and 0.45μm. As the pore size decreases, the membrane binds more firmly to low-molecular-weight proteins. Typically, 0.45μm membranes are used for proteins above 20kDa, while 0.22μm membranes are preferred for proteins below 20kDa.
PVDF membranes are hydrophobic and require pretreatment with methanol before use. This activation step enhances the binding of negatively charged proteins by activating positive charges on the membrane surface. PVDF membranes offer high mechanical strength and can be reused multiple times after treatment with membrane regeneration solution.
Product Information
| Catalog No. | Product Name | Storage Condition | Specification |
|---|---|---|---|
| RA022R | PVDF Membrane (0.22μm) | RT | 30×300cm |
| RA022P | PVDF Membrane (0.22μm) | RT | 6×9cm, 20 sheets |
Storage Condition: Store at room temperature, valid for 5 years.
Application Areas
Common solid support in Western blot experiments
Used in various adsorption analysis experiments
Suitable for amino acid analysis and detection
Used in protein N-terminal sequencing experiments
Ideal choice for Dot and Slot blot experiments
Used for glycoprotein staining analysis
Suitable for lipopolysaccharide analysis experiments
Pore Size Selection Guide
Suitable for proteins above 20kDa
Suitable for low-molecular-weight proteins below 20kDa
Product Advantages
- Tear-Resistant: High mechanical strength, not easily torn;
- High Loading Capacity: Capable of loading more protein samples;
- High Pressure Resistance: Can withstand high voltage;
- High Temperature Resistance: Stable performance under high temperature conditions;
- Reusable: Can be reused multiple times after treatment with membrane regeneration solution.
Precautions
- PVDF membrane needs to be activated with methanol or ethanol;
- This product is for research use only.
