5min Protein-Free Rapid Blocking Solution
Western Rapid Blocking Solution · 5-Minute Blocking · No Washing Required
Product Overview
This product is a ready-to-use protein-free rapid blocking solution that does not contain any protein components. It can be used to block NC or PVDF membranes after transfer. The entire blocking process takes only 5 minutes. The blocking effect is superior to traditional blocking solutions such as non-fat dry milk and BSA, providing better target band signals and lower background results. It has a wide range of applicability and is compatible with WB detection of conventional antibodies and modified antibodies.
Product Information
| Product Name | Storage Condition | Specification 1 | Specification 2 |
|---|---|---|---|
| 5min Protein-Free Rapid Blocking Solution | 2-8℃ | 100mL | 500mL |
| Instruction Manual | - | 1 piece | 1 piece |
Storage Conditions: Store at 2-8℃, shelf life of 18 months.
Time Efficiency Comparison
Unlike other products that require optimization of blocking time, this product can complete blocking in 5 minutes, significantly saving experimental time.
Application Scenarios
Fixed 5-minute blocking time, no need to optimize blocking conditions
Direct primary antibody incubation after blocking, no intermediate washing steps required
Product Advantages
- Low Background: Higher signal-to-noise ratio with clean background;
- High Efficiency: 5-minute rapid blocking;
- Compatibility: Compatible with conventional primary antibodies, as well as horseradish peroxidase (HRP), alkaline phosphatase (AP), and biotin-labeled secondary antibodies;
- Protein-Free: Protein-free design to avoid signal interference;
- No-Wash: Direct primary antibody incubation after blocking.
Operation Procedure
Ready-to-Use Formula: This product is a ready-to-use blocking solution, no dilution required, ready for direct use.
- Place the transferred membrane into an appropriate volume of 5min Protein-Free Rapid Blocking Solution to completely submerge the membrane, and incubate on a shaker at room temperature for 5 minutes;
- After removing the membrane from the blocking solution, directly place it into the primary antibody working solution and proceed with the subsequent antibody incubation process.
Note: The blocked membrane does not need to be washed; washing may affect the blocking effect.
Precautions
- The blocked membrane does not need to be washed; washing may affect the blocking effect;
- The applicability of the blocking solution varies depending on experiments and antibodies; for special experiments, experimental conditions need to be optimized according to specific situations;
- This product is for research use only.
