Product Introduction
There are various ways to stain plant tissues, and their operational procedures are similar to those of animal tissue staining. Saffron O-fixed green staining is the most commonly used staining method in plant sectioning, especially for ordinary sectioning of root, stem, and leaf tissues of higher plants, which can achieve good results.
EnkiLife Safranin O-Fast Green Plant Tissue Staining Solution is mainly composed of Safranin O staining solution and Fast Green staining solution. After staining, in sections of root, stem, leaf and other tissues: Nuclei and lignified cell walls appear bright red; Cutinized cell walls appear translucent pink; Suberized cell walls appear reddish-brown; Cytoplasm and cellulose-containing cell walls appear green. The contrast between the two colors is sharp and easy to observe. Differentiation is critical: over-differentiation may lead to no staining, while under-differentiation may result in overly intense staining.
Basic Information
Product name | Safranin O-Fast Green Plant Tissue Staining Kit |
Sizes | 50 mL |
Storage | RT |
Shipping | RT |
Validity | 12 months |
Product Components
Components | 3x 50mL |
Reagent (A): Safranin O Staining Solution | 50 mL |
Reagent (B): Acid Ethanol Differentiation Solution | 50 mL |
Reagent (C): Fast Green Staining Solution | 50 mL |
Notes
1. After Safranin staining, destaining in 50% ethanol requires optimization. If destaining is insufficient, Fast Green staining will be poor; if excessive, red staining will fade or turn entirely green.
2. Fast Green is a rapid-staining dye. Do not over-stain, or it will cause Safranin to fade.
3. Xylene can be replaced with eco-friendly dewaxing and clearing solution.
4. Staining time is not absolute and may vary with plant species and section thickness.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. Use the reagent promptly after opening to avoid affecting experimental results.
