Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Staphylococcal aureus Enterotoxin Total content in Food samples.
Key features
Sensitivity: 0.15 ppb (ng/mL)
Species reactivity: Universal
Assay time: 120min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Specificity:
SEA | 20% | ||||
SEB | 60% | ||||
SEC | 100% | ||||
SED | 7.5% | ||||
SEE | 20% |
Detection limits:
Raw milk, sterilized milk, pasteurized milk, modified milk, yoghurt | 0.15 ppb | ||||
Milk drink | 0.3 ppb | ||||
Milk powder, whey powder, whey protein powder | 0.9 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This kit uses Sandwich-ELISA as the method for the qualitative detection. It can detect staphylococcal aureus enterotoxin (SET) in samples. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, control and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antibodies. The sample is added to the wells of the ELISA microtiter plate, and the SET in the sample is combined with the pre-coated antibody to form an SET-antibody compound. Free components are washed away. The Antibody Working Solution and HRP Conjugate are added to each well and react with the compound to form “antibody-SET-HRP conjugate” compound. The substrate reagent is added to initiate the color developing reaction. The presence of SET can be determined according to the OD value by using a microplate reader with 450 nm (630 nm) wavelength.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Positive Control | 1 x 2 mL | ||||
Negative Control | 1 x 2 mL | ||||
Yoghurt extract | 1 x 40 mL | ||||
HRP Conjugate | 1 x 12 mL | ||||
Antibody | 1 x 12 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
