Product Introduction
2,3,5-Triphenyltetrazolium chloride (TTC) has a molecular weight of 334.80, molecular formula C19H15ClN4, and CAS number 298-96-4. TTC is a lipophilic, light-sensitive compound originally used to assess seed viability and later applied to visualize ischemic infarction in mammalian tissues. TTC acts as a proton acceptor for the pyridine-nucleotide-linked enzyme system of the respiratory chain. In normal tissue it is reduced by respiratory enzymes to a red product, whereas ischemic tissue, in which respiratory enzyme activity is decreased, remains pale and unchanged. Thus TTC staining provides a macroscopic method for evaluating dehydrogenase activity in tissues.
EnkiLife TTC Staining Solution (1%) permits observation of myocardial infarction and necrotic areas in brain tissue 3–6 h earlier than electron microscopy and 24 h earlier than light microscopy. It is mainly used for fresh heart and brain tissue in autopsy and for staining early infarcted tissue in experimental animal models; it can also be used to assess seed and pollen viability. Product features: 1. Fresh tissue can be stained immediately without fixation, embedding, or other pre-treatment. 2. Short staining time, generally 20–30 min. 3. The solution can be reused; 10 mL can stain about 20 samples.
Basic Information
Product name | TTC Staining Kit, 1% |
Sizes | 100 mL |
Storage | 2-8 ℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 6 months |
Notes
1. TTC Stain (1%) is slightly irritating; handle with care.
2. Maintain brain integrity during dissection.
3. If staining is weak, prolong staining time appropriately.
4. Use the freshest samples possible; enzyme activity in normal myocardium and brain declines quickly—stain promptly.
5. Wear laboratory coat and disposable gloves for personal safety.
6. Use reagent soon after opening to ensure optimal performance.
