Product Introduction
2,3,5-Triphenyltetrazolium chloride (TTC) has a molecular weight of 334.80, molecular formula C19H15ClN4, and CAS number 298-96-4. TTC is a lipophilic, light-sensitive compound originally used to assess seed viability and later applied to visualize ischemic infarction in mammalian tissues. TTC acts as a proton acceptor for the pyridine-nucleotide-linked enzyme system of the respiratory chain. In normal tissue it is reduced by respiratory enzymes to a red product, whereas ischemic tissue, in which respiratory enzyme activity is decreased, remains pale and unchanged. Thus TTC staining provides a macroscopic method for evaluating dehydrogenase activity in tissues.
EnkiLife TTC Staining Solution (4%) is routinely used for fresh cardiac and brain tissues obtained at autopsy and for early infarct visualization in experimental animal models; it can also be employed to determine seed and pollen viability. TTC produces a red formazan upon reaction with dehydrogenases in viable tissue, allowing assessment of tissue or seed viability based on staining location and intensity.
Basic Information
Product name | TTC Staining Kit, 4% |
Sizes | 100 mL |
Storage | 2-8 ℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 6 months |
Notes
1. TTC Stain (4%) is slightly irritating; handle with care.
2. Maintain brain integrity during dissection.
3. If staining is weak, prolong staining time appropriately.
4. Use the freshest samples possible; enzyme activity in normal myocardium and brain declines quickly—stain promptly.
5. Wear laboratory coat and disposable gloves for personal safety.
6. Use reagent soon after opening to ensure optimal performance.
