Product Introduction
Acid phosphatase (ACP) is widely distributed in virtually all tissues, predominantly within lysosomes, and is therefore commonly used as a lysosomal marker enzyme. Extralysosomal ACP is also present in the endoplasmic reticulum and cytoplasm. ACP isoforms differ among species; the optimum pH is 4.5–5.5. Tartrate-resistant acid phosphatase (TRAP) is found in follicles of normal human alveolar macrophages and in leukemic spleen cells, but is not released into blood. Consequently, serum TRAP activity largely reflects osteoclast function, enabling assessment of osteoclastic status via blood measurement.
EnkiLife TRAP Staining Solution uses naphthol AS-BI as substrate; under acidic pH, ACP hydrolyses the substrate to release phosphate and naphthol, which couples with a diazonium salt to yield a colored product localized in the cytoplasm. Cells whose ACP is resistant to tartrate exhibit a positive reaction. Suitable for fresh blood smears, cytospins, and frozen sections.
Basic Information
Product name | Tartrate-Resistant Acid Phosphatase (TRAP) Staining Kit |
Sizes | 10 mL |
Storage | -20 ℃, keep away from light |
Shipping | Shipped with ice pack |
Validity | 6 months |
Product Components
Components | 3x 10mL | |
Reagent (A): TRAP Fixative | 25 mL | |
Reagent (B): TRAP Incubation Solution | B1: AS-BI Buffer | 2 × 0.5 mL |
B2: GBC Stain Solution | 0.5 mL | |
B3: TRAP Buffer | 9 mL | |
Immediately before use, mix B1 : B2 : B3 = 10 : 5 : 90 to prepare TRAP Incubation Solution; use within the same day. | ||
Reagent (C): Lea Hematoxylin Stain Solution | 10 mL | |
Notes
1. TRAP Incubation Solution is prone to deterioration; skin-puncture blood smears are recommended and should be stained soon after drying.
2. Store GBC Stain Solution at 4 °C whenever possible; avoid –20 °C to prevent deliquescence.
3. For frozen sections, minimize exposure to room temperature.
4. Samples must be fresh; process immediately after collection to preserve enzyme activity.
5. Tissue fixation must be carried out at 4 °C and should not exceed 24 h, or enzyme activity will decline or disappear.
6. Use frozen sections; paraffin sections are not suitable.
