Product Introduction
Wright's stain is a composite dye composed of the acidic dye Eosin and the basic dye Methylene Blue, which provides excellent differentiation of protoplasm. Giemsa stain is a mixture of Azure II and Eosin. Its staining principle and results are essentially the same as Wright's method. Giemsa stain has a strong affinity for cytoplasm and effectively demonstrates basophilic characteristics, especially in blood and bone marrow cells. It clearly stains azurophilic, eosinophilic, and basophilic granules. However, it tends to overstain nuclei and poorly reveals nuclear structure. Therefore, Giemsa stain is often used in combination with Wright's stain.
EnkiLife Wright-Giemsa Stain is prepared by grinding and mixing imported Wright's and Giemsa dyes. It produces clear and distinct cellular staining and is commonly used for blood smears, bone marrow smears, and bacterial staining. Cytoplasm appears red, nuclei and bacteria appear blue, and eosinophilic granules appear orange-red. Neutral glycerol is added to the stain to prevent methanol evaporation or oxidation and to enhance the clarity of blood cell staining. This stain consists of a Wright-Giemsa composite staining solution and a phosphate buffer, which can be used either by mixing in equal parts or by treating the specimen sequentially.
Basic Information
Product name | Wright-Giemsa Staining Kit |
Sizes | 100 mL，500 mL |
Storage | RT |
Shipping | RT |
Validity | 24 months |
Product Components
Components | 2x 100mL | 2x 500mL |
Reagent (A): Wright-Giemsa Stain | 100 mL | 500 mL |
Reagent (B): Phosphate Buffer | 100 mL | 500 mL |
Notes
1. Smears should be evenly spread to ensure optimal staining.
2. Do not pour off the stain or rinse the slide directly during staining. Rinse gently from one edge to avoid dye precipitation.
3. The staining solution can be reused, but not repeatedly. Filter before reuse if precipitate is present.
4. If overstaining occurs, destain briefly with methanol or ethanol. Re-staining is not recommended.
5. Adjust staining time or working solution concentration if staining is too weak or too strong.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
