Summary
| Production Name | His-Tag Polyclonal Antibody |
| Application | WB,Elisa |
| Reactivity | Species independent |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Gene Name | His |
| Immunogen | Synthetic peptide:HHHHHH conjugated to KLH. |
| Specificity | The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins. |
| Dilution Ratio | WB 1:5000, ELISA 1:5000-20000, Optimal dilutions should be determined by the end user |
| Host | Rabbit |
| Isotype | IgG |
| Buffer | Liquid in PBS containing 50% glycerol, 2% protective protein and New type preservative N. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Background | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
