HA-Tag Rapid Competitive Assay Kit
For Rapid HA-tagged Protein Detection · Results in 10-15 min · Latex Microsphere Labeled
Product Overview
HA-Tag Rapid Competitive Assay Kit adopts latex microsphere-based immunochromatography based on competitive immunoassay. Red latex microspheres are used as labeling materials for brighter color and lower background. Recombinant HA-Tag protein is immobilized as test line and chicken IgY as control line on the membrane; conjugate pad is coated with latex-labeled anti-HA monoclonal antibody and latex-labeled chicken IgY.
When HA-Tag protein exists in sample, target analyte preferentially binds to labeled antibody and blocks its combination with immobilized antigen. Higher protein concentration leads to fainter test band. Detection range: 0.5~10μg/mL; CV ≤15 for repeatability, relative bias within ±15%, enabling reliable semi-quantitative results.
Key Advantages
Time Comparison with Conventional Methods
Shorten detection period from days down to only 15 minutes!
Product Information
| Cat.No. | Specification | Storage | Shelf Life |
|---|---|---|---|
| RA10088 | 10 Tests | Sealed & Dry at RT | 24 Months |
| RA10088 | 100 Tests | Sealed & Dry at RT | 24 Months |
Components: Individually foil-packed HA-Tag test cards, running buffer, detailed instruction manual
Wide Applications
- Recombinant clone screening: Rapid screening of high-expression positive transformants to save unnecessary experimental cost.
- Induction condition optimization: Compare expression under varied temperature, time and inducer concentrations rapidly.
- Purification tracking: Real-time detection of target protein in loading, flow-through and eluate fractions.
- Transfection efficiency assay: Evaluate different transfection reagents and experimental protocols conveniently.
- Protein QC verification: Confirm tag integrity for subsequent functional research.
- Industrial production QC: Intermediate testing for recombinant biologic drugs and industrial enzymes.
Operating Procedure
- Preparation: Take test card from foil pouch and lay flat; equilibrate refrigerated samples to ambient temperature.
- Sample Pretreatment: Transfer 80μL running buffer into tube, add 20μL sample and vortex for 10s.
- Sample Loading: Pipette 70μL mixed solution into sample well slowly.
- Result Reading: Incubate 10-15min at room temperature before recording data.
Dilution Guide: Dilute known-concentration samples to ~5μg/mL; dilute unknown samples properly according to expression system and re-dilute if beyond linear range.
Low-abundance Tip: Undiluted raw sample can be used; always run identical matrix negative control for band comparison.
Result Interpretation
Both Control(C) and Test(T) develop red bands with identical intensity vs negative control; no HA-Tag or concentration below 0.5μg/mL LOD.
Control(C) shows red band while Test(T) is absent or markedly weaker than control; paler T band means higher HA-Tag concentration.
No red band on Control(C) regardless of Test(T); invalid caused by expired reagent, wrong operation or insufficient sample volume.
Compatible Common Reagents
Good tolerance to regular lab buffers, direct testing without extra sample treatment.
| Reagent Name | Max Concentration |
|---|---|
| NaCl | 0.5M |
| KCl | 0.5M |
| Urea | 0.4M |
| Triton X-100 | 1% |
| Tween-20 | 1% |
| Imidazole | 0.5M |
| Reagent Name | Max Concentration |
|---|---|
| NP-40 | 1% |
| CHAPS | 1% |
| SDS | 0.2% |
| EDTA | 5mM |
| Glycerol | 10% |
| RIPA | 100% |
Precautions
- For research only, forbidden for human clinical diagnosis or therapy.
- Single-use product, do not reuse test cards.
- Semi-quantitative kit, not for absolute precise quantification.
- Keep stationary during incubation without shaking.
- Never mix components from different production batches.
- Use buffer and test strips immediately after opening package.
- Discard experimental waste per laboratory biosafety standards.
Troubleshooting
| Phenomenon | Possible Causes | Solutions |
|---|---|---|
| No obvious T line fading vs negative control | No target protein; concentration below detection limit | Verify vector matching; test undiluted sample with negative control |
| Faint or fully missing Test band | Concentration exceeds upper working limit; complete competitive inhibition | Dilute sample at higher fold and repeat test |
| No Control band visible | Wrong operation; expired; improper storage; insufficient sample added | Follow manual strictly; replace new card; store sealed dry; load exact 70μL sample |
