Antibody Stripping Buffer (Membrane Regeneration Solution)
Specialized for Western Blot Membrane Regeneration · 10-Minute Fast Stripping · Protects Membrane-Bound Proteins
Gentle Formula 10-Minute Fast Stripping One Membrane Reusable 3-5 Times
Product Overview
This product adopts a gentle acidic washing formula without irritating organic reagents such as β-mercaptoethanol. It efficiently removes antibodies from PVDF or NC membranes in 10 minutes with almost no stripping of membrane-bound proteins. The same membrane can be subjected to 3-5 rounds of antibody detection without repeated electrophoresis and membrane transfer, saving samples and time.
Core Advantages
10-Minute Fast Stripping
Complete removal of membrane-bound antibodies in just 10 minutes, saving significant time compared to traditional methods.
Gentle Formula Protects Proteins
The gentle acidic formula only removes antibodies with almost no stripping of membrane-bound proteins, ensuring the accuracy of subsequent detection.
Membrane Reusable
The same membrane can be used for 3-5 rounds of antibody detection without repeated electrophoresis and membrane transfer, saving samples and reagents.
Time Comparison with Traditional Methods
Traditional Method
Re-Electrophoresis60-90 minutes
Membrane Transfer30-90 minutes
Sample Preparation30 minutes
Total: 2-3.5 hours
Using Our Product
Antibody Stripping10 minutes
Washing15-30 minutes
Re-Blocking5-10 minutes
Total: 30-50 minutes
Using this product can shorten membrane regeneration time from 2-3.5 hours to only 30-50 minutes, improving efficiency by 3-4 times!
Product Specifications
| Product Name | Storage Condition | Specification 1 | Specification 2 |
|---|---|---|---|
| Antibody Stripping Buffer | 2-8℃ | 100mL | 500mL |
Storage Condition: Store at 2-8℃, shelf life of 1 year.
Product Features
- Efficient Stripping: Obtain a "new" membrane in 10 minutes;
- Low Loss: Almost no loss of membrane-bound proteins;
- Odorless: No irritating odor;
- Repeated Stripping: One membrane can be stripped more than 3 times.
Usage Process
1
Stripping
Immerse the membrane in stripping buffer and incubate on a shaker at room temperature for 10 minutes
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2
Washing
Wash 3 times with TBST or PBST, 5-10 minutes each time
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3
Detection (Optional)
Detect stripping effect, repeat steps 1-2 if necessary
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4
Re-Blocking
Re-block and proceed to the next round of Western Blot experiment
- Stripping: Immerse the blotted membrane after exposure into an appropriate amount of antibody stripping buffer (sufficient to completely submerge the membrane), and incubate on a shaker at room temperature for 10 minutes;
- Washing 1: Take out the stripped membrane and wash it 3 times with washing buffer (TBST or PBST), 5-10 minutes each time;
- Detection of Stripping Effect (Optional): Directly expose and develop the membrane from step 2 to detect. If signals still exist, repeat steps 1 and 2 until no signals are detected on the membrane;
- Washing 2 (Optional): Take out the membrane from step 3 and wash it 3 times with washing buffer (TBST or PBST), 5-10 minutes each time;
- Blocking: Re-block the washed membrane and proceed to a new round of Western Blot immunoassay experiment.
Precautions
- It is recommended to detect low-abundance proteins first, then high-abundance proteins;
- This product is for research use only.
