10×TBST Washing Buffer
Specialized for Western Blot Washing · Multipurpose Immunoassay Buffer
Product Overview
This product can be used in Western Blot experiments to wash away non-specifically bound antibodies and other reagents on the membrane, as well as in immunofluorescence, immunohistochemistry and other experiments for the preparation of blocking solution, preparation of primary or secondary antibodies, and washing after primary or secondary antibody incubation, so as to reduce background and enhance signal-to-noise ratio.
This product is a 10× concentrate with main components: 100 mM Tris, 1.5 M NaCl, 0.5% Tween-20. It needs to be diluted 10 times before use.
Application Areas
Wash away non-specifically bound antibodies and other reagents on the membrane
Blocking solution preparation, washing after antibody incubation
Primary or secondary antibody preparation, washing steps
Product Specifications
| Product Name | Storage Condition | Specification |
|---|---|---|
| 10×TBST | RT | 1L |
Storage Condition: Store at room temperature, shelf life of 1 year.
Usage Instructions
Take 100 mL of this product and mix thoroughly with 900 mL of deionized water, distilled water, or pure water to obtain 1×TBST working solution containing 10 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.2-7.6.
Product Features
- Multipurpose: Suitable for Western Blot, immunofluorescence, immunohistochemistry and other immunoassay experiments;
- Efficient Washing: Effectively removes non-specific binding, reduces background, and enhances signal-to-noise ratio;
- Concentrated Formula: 10× concentrate saves storage space and is easy to use;
- Stable Formula: Main components are 100 mM Tris, 1.5 M NaCl, 0.5% Tween-20 with stable performance;
- Easy to Use: Can be used by diluting at a ratio of 1:9, simple operation.
Precautions
- After diluting this product into 1× working solution, it is recommended to store at 2~8℃ and use within one week;
- If crystals precipitate in the product, place it in a 37℃ water bath until completely dissolved, which does not affect use;
- This product is for research use only.
