Western Blot (WB) Workflow & Product Solution Atlas
Complete experimental workflow from sample preparation to result analysis, featuring key reagents at each step and integrated solutions.
Stage 1: Sample Preparation
Cell/tissue lysis and protein extraction/quantification
1. Sample Lysis and Protein Extraction
Efficiently lyse cells or tissues to release intracellular proteins while maintaining protein integrity and biological activity.
Cell/Tissue Lysis Buffer
"Lyse all samples, zero protein degradation"
- Efficient extraction of high molecular weight proteins while preserving protein integrity
- Protects post-translational modifications
- Ready-to-use; no need for additional protease inhibitors
- Compatible with both cell and tissue samples; broad applicability
RIPA Lysis Buffer (strong/medium/weak)
"Selectable strength, precise lysis"
- Three strength options to meet different experimental needs
- Strong cell lysis, high total protein extraction efficiency
- Preserves protein activity, suitable for subsequent assays
- Suitable for a variety of cell and tissue samples
2. Protein Concentration Determination
Accurately determine protein sample concentration to ensure precise protein loading for subsequent experiments.
Protein Assay Kit (BCA Method)
"Accurate quantification, worry-free experimentation"
- BCA method principle, high accuracy, good reproducibility
- Wide linear range, adaptable to samples of different concentrations
- Easy to operate, fast results
- Strong compatibility, suitable for multiple sample types
Stage 2: Gel Electrophoresis
Protein separation and gel preparation
3. Gel Preparation
Prepare polyacrylamide gels for protein separation based on molecular weight.
RapidSet Broad-Range Gradient Gel Kit
"Gel kit that doesn't pick on molecular weight"
- 4-20% linear gradient, wide separation range
- Ready-to-use design, easy to operate
- Fast polymerization, saves time
- Consistent batch-to-batch performance, reproducible results
Bis-Tris Precast Gel (4-20%)
"Stable at medium voltage, fast at high voltage – take the mystery out of gel casting"
- 4-20% linear gradient, wide separation range
- Bis-Tris buffer system, higher resolution
- Precast gel design, saves gel preparation time
- 12/15 well design, meets high-throughput needs
4. Marker Selection and Loading Reference
Provide precise molecular weight reference for real-time monitoring of electrophoresis progress, assessing transfer efficiency, and accurate target protein molecular weight determination.
Calibrated Color Prestained Protein Marker (8-180kDa / 10-250kDa)
"Making standards more standard"
- Broad coverage, precise reference: Covers commonly used ranges of 8-180kDa and 10-250kDa; key molecular weight bands deviate<2%, accuracy comparable to non-prestained markers
- Color design, real-time monitoring: Characteristic colors at 72kDa (orange-red), 8/25kDa (green), etc., allowing real-time observation of progress and efficiency after electrophoresis and transfer without staining
- Excellent stability: Tested after 50 freeze-thaw cycles, 40°C high temperature, and room temperature storage; stable performance, addressing concerns about transport, storage, and frequent use
- Extremely simple operation: Pre-mixed with 1× loading buffer; no boiling or dilution needed; direct loading improves experimental efficiency
Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa)
"Precision ruler for large molecule research"
- Specifically designed for large molecules: Covers a broad range of 25-400kDa, precisely covering the majority of research large molecular weight proteins (e.g., >200kDa proteins)
- High accuracy and clarity: Key high molecular weight bands (e.g., 250kDa, 400kDa) migrate accurately; loading 5-10μL yields sharp, non-smearing bands
- Cross-system compatibility: Maintains stable migration in various electrophoresis buffer systems such as Tris-Glycine, Tricine, Bis-Tris
- Visual transfer validation: Colored bands allow direct confirmation of successful transfer of large molecular weight proteins after transfer, eliminating Ponceau S staining and avoiding antibody waste
5. Sample Preparation and Loading
Mix protein samples with loading buffer, denature, and load into gel wells for electrophoresis.
5×Loading Buffer
"Non-toxic, low viscosity, worry-free experimentation"
- Non-toxic formula, safer experimental operation
- Low viscosity, uniform loading without residue
- 5× concentrate, saves storage space
- Compatible with various protein detection methods
6. Electrophoresis Separation
Under an electric field, proteins migrate and separate in the gel based on molecular weight.
20× Universal Rapid Electrophoresis Buffer
"The universal 'stimulant' for electrophoresis experiments"
- 20× concentrate, saves storage space
- Universal formula, compatible with multiple systems
- Fast electrophoresis, saves experimental time
- Strong buffering capacity, clear bands
Stage 3: Transfer
Protein transfer to membrane
7. Protein Transfer
Transfer separated proteins from the gel to a membrane for subsequent immunodetection.
20× Ice-Free Rapid Transfer Buffer
"Methanol-free, reusable, safe and twice as fast"
- Methanol-free formula, safer to use
- 20× concentrate, saves storage space
- Fast transfer, no ice bath required
- Reusable, economical and environmentally friendly
Stage 4: Immunodetection
Blocking, antibody incubation, and washing
8. Membrane Blocking
Block non-specific binding sites on the membrane to reduce background signal.
1min Protein-Free Rapid Blocking Solution
"Effortless blocking, works no matter how you rush"
- 1-minute rapid blocking, ultra-fast experiment
- Protein-free formula, avoids cross-reaction
- Extremely low background, improves signal-to-noise ratio
- Suitable for various detection systems
5min Protein-Free Rapid Blocking Solution
"Protein-free, yet background remains white"
- 5-minute rapid blocking, saves time
- Protein-free formula, avoids cross-contamination
- Extremely low background, high signal-to-noise ratio
- Suitable for fluorescence and chemiluminescence detection
5× Protein-Free Rapid Blocking Diluent
"Two-in-one blocking and dilution, suitable for both primary and secondary antibodies"
- Two-in-one blocking and dilution function
- 5× concentrate, saves storage space
- Protein-free formula, reduces background
- Suitable for primary and secondary antibody dilution
9. Antibody Incubation
Specific binding of primary and secondary antibodies to the target protein.
No-Blocking Rapid Antibody Diluent
"One less blocking step, one step faster incubation"
- No-blocking design, saves experimental time
- Primary and secondary antibody incubation completed in 20 minutes
- Reduces operational steps, minimizes errors
- Maintains high signal-to-noise ratio, clean background
10. Membrane Washing
Remove unbound and non-specifically bound antibodies to reduce background signal.
10×TBST
"Time is precious, washing buffer saves you 10x"
- 10× concentrate, saves storage space
- Concentrated formula, dilute as needed, flexible use
- Optimized Tween concentration, more thorough washing
- Stable pH, minimal batch-to-batch variation
Stage 5: Detection and Analysis
Signal detection and result analysis
11. Signal Detection
Detect target protein signal using chemiluminescence or fluorescence methods.
Super-sensitive ECL chemiluminescent reagent
"Femtogram-level detection, ultra-sensitive"
- Femtogram-level ultra-high sensitivity, low detection limit
- Strong and stable signal, long duration
- Low background, high signal-to-noise ratio
- Suitable for low-abundance protein detection
12. Membrane Stripping and Re-probing
Remove bound antibodies for detection of other proteins.
Antibody Stripping Buffer
"Efficiently strip antibodies, retain membrane-bound proteins, make stripping as simple as washing your face"
- Efficiently strips antibodies while retaining membrane-bound proteins
- Supports multiple rounds of detection on the same membrane
- Simple operation, incubate at room temperature
- Does not damage PVDF/NC membranes
Stage 6: Auxiliary Detection
Total protein detection and staining
13. Total Protein Staining
Detect total protein loading amount as a loading control.
Coomassie Brilliant Blue Fast Staining Solution (Non-Dehydration)
"Color development in 2 minutes, staining that really 'hits the mark'"
- Fast color development in 2 minutes, saves experimental time
- Non-dehydration design, easier operation
- Clean staining background, clear bands
- High sensitivity, low detection limit
Stage 7: Complete Solutions
We offer full-workflow solutions with various durations and features. You can choose based on the urgency of your experiment and requirements for sensitivity and reproducibility.
14. Complete Workflow Kits
Complete Western Blot experiment all-in-one solutions, meeting different experimental needs from ultra-fast to routine.
2-Hour Rapid Western Blot Ready-to-Use Complete Workflow Kit
"Complete in 2 hours, CV<5%"
- Ultra-fast and efficient: Complete workflow in approximately 2 hours, 5-10x faster
- Reliable results: Excellent reproducibility, band intensity CV<5%, minimal inter-experiment variability
- Rapid incubation technology: Blocking 10 min, primary antibody 15 min, secondary antibody 5 min
- Safe and eco-friendly: Ready-to-use precast gels, odor-free buffers, avoid contact with toxic reagents
4-Hour Fast Western Blot Ready-to-Use Complete Workflow Kit
"300% efficiency boost, fg-level sensitivity"
- Efficient and fast: Total time only 3.6-4 hours, saving 85% time compared to traditional methods
- Ready-to-use convenience: Contains most reagents and consumables, reduces experiment preparation time by 80%
- Excellent performance: fg-level high sensitivity, extremely low background, clear and sharp bands
- Beginner-friendly: Standardized process, significantly reduces operational difficulty and human error
Streamlined Western Blot Complete Kit
"Complete WB workflow in 5 hours"
- Complete solution: Full workflow from gel preparation to development
- Fast completion: Optimized process completes the entire WB experiment within 5 hours
- One-stop: Contains all necessary reagents, one-stop shopping
- Stable and reliable: Optimized formula, easy to operate, suitable for routine testing